Regulation of surfactant protein D in the mouse female reproductive tract in vivo

Rebecca E. Oberley, Kelli L. Goss, Darren S. Hoffmann, Kevin A. Ault, Traci L. Neff, Kyle H. Ramsey, Jeanne M. Snyder

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Surfactant protein D (SP-D) plays a role in innate immunity in the lung and is expressed at many other mucosal surfaces throughout the human body. In this study, we show that SP-D mRNA and protein are present in the murine female reproductive tract; i.e. in the vagina, cervix, uterus and oviduct. SP-D protein is primarily localized to epithelial cells lining the genital tract and is also present in secretory material within the lumen of the uterus and cervix. The levels of SP-D mRNA in the uterus vary by a factor of 10 during the estrous cycle with peak levels present at estrus and the lowest levels at diestrus. In contrast, SP-D mRNA levels in the lung do not change during the estrous cycle. Since SP-D is an innate host defense protein present in the mouse reproductive tract, we studied the influence of infection on SP-D levels in vivo. We found that Chlamydia muridarum infection caused an increase in the SP-D protein content of reproductive tract epithelial cells. These data are suggestive that SP-D may play a role in innate immunity in the female reproductive tract in vivo.

Original languageEnglish (US)
Pages (from-to)863-868
Number of pages6
JournalMolecular Human Reproduction
Volume13
Issue number12
DOIs
StatePublished - Dec 1 2007

Fingerprint

Pulmonary Surfactant-Associated Protein D
Estrous Cycle
Innate Immunity
Cervix Uteri
Messenger RNA
Chlamydia muridarum
Proteins
Epithelial Cells
Diestrus
Lung
Chlamydia Infections
Oviducts
Estrus
Vagina
Human Body
Uterus

Keywords

  • Female reproductive tract
  • Infection
  • Mouse
  • Regulation
  • SP-D

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Cell Biology

Cite this

Oberley, R. E., Goss, K. L., Hoffmann, D. S., Ault, K. A., Neff, T. L., Ramsey, K. H., & Snyder, J. M. (2007). Regulation of surfactant protein D in the mouse female reproductive tract in vivo. Molecular Human Reproduction, 13(12), 863-868. https://doi.org/10.1093/molehr/gam074

Regulation of surfactant protein D in the mouse female reproductive tract in vivo. / Oberley, Rebecca E.; Goss, Kelli L.; Hoffmann, Darren S.; Ault, Kevin A.; Neff, Traci L.; Ramsey, Kyle H.; Snyder, Jeanne M.

In: Molecular Human Reproduction, Vol. 13, No. 12, 01.12.2007, p. 863-868.

Research output: Contribution to journalArticle

Oberley, RE, Goss, KL, Hoffmann, DS, Ault, KA, Neff, TL, Ramsey, KH & Snyder, JM 2007, 'Regulation of surfactant protein D in the mouse female reproductive tract in vivo', Molecular Human Reproduction, vol. 13, no. 12, pp. 863-868. https://doi.org/10.1093/molehr/gam074
Oberley, Rebecca E. ; Goss, Kelli L. ; Hoffmann, Darren S. ; Ault, Kevin A. ; Neff, Traci L. ; Ramsey, Kyle H. ; Snyder, Jeanne M. / Regulation of surfactant protein D in the mouse female reproductive tract in vivo. In: Molecular Human Reproduction. 2007 ; Vol. 13, No. 12. pp. 863-868.
@article{8aa7681046694df79d39c9ec27662e81,
title = "Regulation of surfactant protein D in the mouse female reproductive tract in vivo",
abstract = "Surfactant protein D (SP-D) plays a role in innate immunity in the lung and is expressed at many other mucosal surfaces throughout the human body. In this study, we show that SP-D mRNA and protein are present in the murine female reproductive tract; i.e. in the vagina, cervix, uterus and oviduct. SP-D protein is primarily localized to epithelial cells lining the genital tract and is also present in secretory material within the lumen of the uterus and cervix. The levels of SP-D mRNA in the uterus vary by a factor of 10 during the estrous cycle with peak levels present at estrus and the lowest levels at diestrus. In contrast, SP-D mRNA levels in the lung do not change during the estrous cycle. Since SP-D is an innate host defense protein present in the mouse reproductive tract, we studied the influence of infection on SP-D levels in vivo. We found that Chlamydia muridarum infection caused an increase in the SP-D protein content of reproductive tract epithelial cells. These data are suggestive that SP-D may play a role in innate immunity in the female reproductive tract in vivo.",
keywords = "Female reproductive tract, Infection, Mouse, Regulation, SP-D",
author = "Oberley, {Rebecca E.} and Goss, {Kelli L.} and Hoffmann, {Darren S.} and Ault, {Kevin A.} and Neff, {Traci L.} and Ramsey, {Kyle H.} and Snyder, {Jeanne M.}",
year = "2007",
month = "12",
day = "1",
doi = "10.1093/molehr/gam074",
language = "English (US)",
volume = "13",
pages = "863--868",
journal = "Molecular Human Reproduction",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "12",

}

TY - JOUR

T1 - Regulation of surfactant protein D in the mouse female reproductive tract in vivo

AU - Oberley, Rebecca E.

AU - Goss, Kelli L.

AU - Hoffmann, Darren S.

AU - Ault, Kevin A.

AU - Neff, Traci L.

AU - Ramsey, Kyle H.

AU - Snyder, Jeanne M.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Surfactant protein D (SP-D) plays a role in innate immunity in the lung and is expressed at many other mucosal surfaces throughout the human body. In this study, we show that SP-D mRNA and protein are present in the murine female reproductive tract; i.e. in the vagina, cervix, uterus and oviduct. SP-D protein is primarily localized to epithelial cells lining the genital tract and is also present in secretory material within the lumen of the uterus and cervix. The levels of SP-D mRNA in the uterus vary by a factor of 10 during the estrous cycle with peak levels present at estrus and the lowest levels at diestrus. In contrast, SP-D mRNA levels in the lung do not change during the estrous cycle. Since SP-D is an innate host defense protein present in the mouse reproductive tract, we studied the influence of infection on SP-D levels in vivo. We found that Chlamydia muridarum infection caused an increase in the SP-D protein content of reproductive tract epithelial cells. These data are suggestive that SP-D may play a role in innate immunity in the female reproductive tract in vivo.

AB - Surfactant protein D (SP-D) plays a role in innate immunity in the lung and is expressed at many other mucosal surfaces throughout the human body. In this study, we show that SP-D mRNA and protein are present in the murine female reproductive tract; i.e. in the vagina, cervix, uterus and oviduct. SP-D protein is primarily localized to epithelial cells lining the genital tract and is also present in secretory material within the lumen of the uterus and cervix. The levels of SP-D mRNA in the uterus vary by a factor of 10 during the estrous cycle with peak levels present at estrus and the lowest levels at diestrus. In contrast, SP-D mRNA levels in the lung do not change during the estrous cycle. Since SP-D is an innate host defense protein present in the mouse reproductive tract, we studied the influence of infection on SP-D levels in vivo. We found that Chlamydia muridarum infection caused an increase in the SP-D protein content of reproductive tract epithelial cells. These data are suggestive that SP-D may play a role in innate immunity in the female reproductive tract in vivo.

KW - Female reproductive tract

KW - Infection

KW - Mouse

KW - Regulation

KW - SP-D

UR - http://www.scopus.com/inward/record.url?scp=37549069698&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=37549069698&partnerID=8YFLogxK

U2 - 10.1093/molehr/gam074

DO - 10.1093/molehr/gam074

M3 - Article

C2 - 17954522

AN - SCOPUS:37549069698

VL - 13

SP - 863

EP - 868

JO - Molecular Human Reproduction

JF - Molecular Human Reproduction

SN - 1360-9947

IS - 12

ER -