Regulation of miRNA abundance by RNA binding protein TOUGH in Arabidopsis

Guodong Ren, Meng Xie, Yongchao Dou, Shuxin Zhang, Chi Zhang, Bin Yu

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are regulators of gene expression in plants and animals. The biogenesis of miRNAs is precisely controlled to secure normal development of organisms. Here we report that TOUGH (TGH) is a component of the DCL1-HYL1-SERRATE complex that processes primary transcripts of miRNAs [i.e., primary miRNAs (pri-miRNAs)] into miRNAs in Arabidopsis. Lack of TGH impairs multiple DCL activities in vitro and reduces the accumulation of miRNAs and siRNAs in vivo. TGH is an RNA-binding protein, binds pri-miRNAs and precursor miRNAs in vivo, and contributes to pri-miRNA-HYL1 interaction. These results indicate that TGH might regulate abundance of miRNAs through promoting DCL1 cleavage efficiency and/or recruitment of pri-miRNAs.

Original languageEnglish (US)
Pages (from-to)12817-12821
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number31
DOIs
StatePublished - Jul 31 2012

Fingerprint

RNA-Binding Proteins
MicroRNAs
Arabidopsis
Regulator Genes
Gene Expression

ASJC Scopus subject areas

  • General

Cite this

Regulation of miRNA abundance by RNA binding protein TOUGH in Arabidopsis. / Ren, Guodong; Xie, Meng; Dou, Yongchao; Zhang, Shuxin; Zhang, Chi; Yu, Bin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 31, 31.07.2012, p. 12817-12821.

Research output: Contribution to journalArticle

@article{c164342840174138894abd18a62ca294,
title = "Regulation of miRNA abundance by RNA binding protein TOUGH in Arabidopsis",
abstract = "MicroRNAs (miRNAs) are regulators of gene expression in plants and animals. The biogenesis of miRNAs is precisely controlled to secure normal development of organisms. Here we report that TOUGH (TGH) is a component of the DCL1-HYL1-SERRATE complex that processes primary transcripts of miRNAs [i.e., primary miRNAs (pri-miRNAs)] into miRNAs in Arabidopsis. Lack of TGH impairs multiple DCL activities in vitro and reduces the accumulation of miRNAs and siRNAs in vivo. TGH is an RNA-binding protein, binds pri-miRNAs and precursor miRNAs in vivo, and contributes to pri-miRNA-HYL1 interaction. These results indicate that TGH might regulate abundance of miRNAs through promoting DCL1 cleavage efficiency and/or recruitment of pri-miRNAs.",
author = "Guodong Ren and Meng Xie and Yongchao Dou and Shuxin Zhang and Chi Zhang and Bin Yu",
year = "2012",
month = "7",
day = "31",
doi = "10.1073/pnas.1204915109",
language = "English (US)",
volume = "109",
pages = "12817--12821",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "31",

}

TY - JOUR

T1 - Regulation of miRNA abundance by RNA binding protein TOUGH in Arabidopsis

AU - Ren, Guodong

AU - Xie, Meng

AU - Dou, Yongchao

AU - Zhang, Shuxin

AU - Zhang, Chi

AU - Yu, Bin

PY - 2012/7/31

Y1 - 2012/7/31

N2 - MicroRNAs (miRNAs) are regulators of gene expression in plants and animals. The biogenesis of miRNAs is precisely controlled to secure normal development of organisms. Here we report that TOUGH (TGH) is a component of the DCL1-HYL1-SERRATE complex that processes primary transcripts of miRNAs [i.e., primary miRNAs (pri-miRNAs)] into miRNAs in Arabidopsis. Lack of TGH impairs multiple DCL activities in vitro and reduces the accumulation of miRNAs and siRNAs in vivo. TGH is an RNA-binding protein, binds pri-miRNAs and precursor miRNAs in vivo, and contributes to pri-miRNA-HYL1 interaction. These results indicate that TGH might regulate abundance of miRNAs through promoting DCL1 cleavage efficiency and/or recruitment of pri-miRNAs.

AB - MicroRNAs (miRNAs) are regulators of gene expression in plants and animals. The biogenesis of miRNAs is precisely controlled to secure normal development of organisms. Here we report that TOUGH (TGH) is a component of the DCL1-HYL1-SERRATE complex that processes primary transcripts of miRNAs [i.e., primary miRNAs (pri-miRNAs)] into miRNAs in Arabidopsis. Lack of TGH impairs multiple DCL activities in vitro and reduces the accumulation of miRNAs and siRNAs in vivo. TGH is an RNA-binding protein, binds pri-miRNAs and precursor miRNAs in vivo, and contributes to pri-miRNA-HYL1 interaction. These results indicate that TGH might regulate abundance of miRNAs through promoting DCL1 cleavage efficiency and/or recruitment of pri-miRNAs.

UR - http://www.scopus.com/inward/record.url?scp=84864517927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864517927&partnerID=8YFLogxK

U2 - 10.1073/pnas.1204915109

DO - 10.1073/pnas.1204915109

M3 - Article

C2 - 22802657

AN - SCOPUS:84864517927

VL - 109

SP - 12817

EP - 12821

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 31

ER -