Regulation of hyphal morphogenesis and the DNA damage response by the Aspergillus nidulans ATM homolog AtmA

Iran Malavazi, Camile P. Semighini, Marcia Regina Von Zeska Kress, Steven D Harris, Gustavo H. Goldman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ataxia telangiectasia (A-T) is an inherited disorder characterized by progressive loss of motor function and susceptibility to cancer. The most prominent clinical feature observed in A-T patients is the degeneration of Purkinje motor neurons. Numerous studies have emphasized the role of the affected gene product, ATM, in the regulation of the DNA damage response. However, in Purkinje cells, the bulk of ATM localizes to the cytoplasm and may play a role in vesicle trafficking. The nature of this function, and its involvement in the pathology underlying A-T, remain unknown. Here we characterize the homolog of ATM (AtmA) in the filamentous fungus Aspergillus nidulans. In addition to its expected role in the DNA damage response, we find that AtmA is also required for polarized hyphal growth. We demonstrate that an atmA mutant fails to generate a stable axis of hyphal polarity. Notably, cytoplasmic microtubules display aberrant cortical interactions at the hyphal tip. Our results suggest that AtmA regulates the function and/or localization of landmark proteins required for the formation of a polarity axis. We propose that a similar function may contribute to the establishment of neuronal polarity.

Original languageEnglish (US)
Pages (from-to)99-109
Number of pages11
JournalGenetics
Volume173
Issue number1
DOIs
StatePublished - May 1 2006

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Aspergillus nidulans
Ataxia Telangiectasia
Morphogenesis
DNA Damage
Purkinje Cells
Motor Neurons
Microtubules
Cytoplasm
Fungi
Pathology
Growth
Genes
Neoplasms
Proteins

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Malavazi, I., Semighini, C. P., Von Zeska Kress, M. R., Harris, S. D., & Goldman, G. H. (2006). Regulation of hyphal morphogenesis and the DNA damage response by the Aspergillus nidulans ATM homolog AtmA. Genetics, 173(1), 99-109. https://doi.org/10.1534/genetics.105.052704

Regulation of hyphal morphogenesis and the DNA damage response by the Aspergillus nidulans ATM homolog AtmA. / Malavazi, Iran; Semighini, Camile P.; Von Zeska Kress, Marcia Regina; Harris, Steven D; Goldman, Gustavo H.

In: Genetics, Vol. 173, No. 1, 01.05.2006, p. 99-109.

Research output: Contribution to journalArticle

Malavazi, I, Semighini, CP, Von Zeska Kress, MR, Harris, SD & Goldman, GH 2006, 'Regulation of hyphal morphogenesis and the DNA damage response by the Aspergillus nidulans ATM homolog AtmA', Genetics, vol. 173, no. 1, pp. 99-109. https://doi.org/10.1534/genetics.105.052704
Malavazi, Iran ; Semighini, Camile P. ; Von Zeska Kress, Marcia Regina ; Harris, Steven D ; Goldman, Gustavo H. / Regulation of hyphal morphogenesis and the DNA damage response by the Aspergillus nidulans ATM homolog AtmA. In: Genetics. 2006 ; Vol. 173, No. 1. pp. 99-109.
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