Regulation of guinea pig very low density lipoprotein secretion rates by dietary fat saturation

Ghada Soliman, M. L. Fernandez, D. J. McNamara

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

We investigated the effects of dietary fat saturation on very low density lipoprotein (VLDL) production in guinea pigs fed semipurified diets containing 15% (w/w) fat, either corn oil (CO, 58% linoleic acid), lard (LA, 42% oleic and 24% palmitic acids) or palm kernel oil (PK, 52% lauric and 18% myristic acids) for 4 weeks. Animals were given an intravenous injection of Triton WR 1339 to block VLDL catabolism and rates of VLDL, triacylglycerol (TAG) and apolipoprotein (apo) B secretion were measured over time. Plasma TAG concentrations increased linearly for 8 h (r = 0.99) and VLDL-TAG secretion rates were significantly higher (P < 0.01) in guinea pigs fed LA (72.7 ± 14.7 mg/kg-h, n = 12) compared to animals fed PK (55.4 ± 13.4 mg/kg-h, n = 12) or CO (48.6 ± 17.5 mg/kg-h, n = 5). VLDL apoB secretion rates were highest in PK-fed animals (3.1 ± 1.8 mg/kg-h) compared to guinea pigs fed LA (1.5 ± 0.8 mg/kg-h) or CO (1.1 ± 0.6 mg/kg-h) diets (P < 0.005). Concurrent with analysis of VLDL secretion, turnover of 125I- labeled LDL was measured. Low density lipoprotein (LDL) fractional catabolic rates were not altered by Triton treatment and LDL apoB specific radioactivity (cpm/μg) did not change time indicating that: a) the Triton blockage of VLDL catabolism was complete, and b) there was no direct secretion of LDL by the liver. These data demonstrate that intake of lard increases the rate of VLDL-triacylglycerol secretion and that nascent VLDL particles from the lard and corn oil diet groups have the same relative triacylglycerol content, whereas palm kernel oil intake increases secretion of VLDL particles which have a reduced triacylglycerol content. These results demonstrate that dietary fat chain length and saturation have specific effects on VLDL secretion rates affecting both particle number and composition.

Original languageEnglish (US)
Pages (from-to)1188-1198
Number of pages11
JournalJournal of Lipid Research
Volume36
Issue number6
StatePublished - Jan 1 1995

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VLDL Lipoproteins
Dietary Fats
Guinea Pigs
Triglycerides
LDL Lipoproteins
Apolipoproteins B
Carbon Monoxide
Nutrition
Animals
Corn Oil
Diet
Myristic Acids
Palmitic Acids
Radioactivity
Linoleic Acid
Chain length
Intravenous Injections
Liver
Fats
Plasmas

Keywords

  • apolipoprotein B
  • low density lipoproteins
  • triacylglycerol secretion

ASJC Scopus subject areas

  • Endocrinology

Cite this

Regulation of guinea pig very low density lipoprotein secretion rates by dietary fat saturation. / Soliman, Ghada; Fernandez, M. L.; McNamara, D. J.

In: Journal of Lipid Research, Vol. 36, No. 6, 01.01.1995, p. 1188-1198.

Research output: Contribution to journalArticle

Soliman, G, Fernandez, ML & McNamara, DJ 1995, 'Regulation of guinea pig very low density lipoprotein secretion rates by dietary fat saturation', Journal of Lipid Research, vol. 36, no. 6, pp. 1188-1198.
Soliman, Ghada ; Fernandez, M. L. ; McNamara, D. J. / Regulation of guinea pig very low density lipoprotein secretion rates by dietary fat saturation. In: Journal of Lipid Research. 1995 ; Vol. 36, No. 6. pp. 1188-1198.
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