Regulation of gene expression of myeloperoxidase during myeloid differentiation

Andreas Tobler, Carl W. Miller, Keith R. Johnson, Michael E. Selsted, Giovanni Rovera, H. Phillip Koeffler

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Myeloperoxidase (MPO) is a major heme enzyme involved in inflammatory responses of polymorphonuclear leukocytes. Using cDNA and intron specific probes for MPO we studied the regulation of MPO expression during myeloid differentiation of the promyelocytic HL‐60 leukemia cell line. Mature MPO mRNA species of 3.3, 2.8 and 1.6 kb and heterogenous nuclear (hn)RNA of > 8 and ∼4 kb were observed in wildtype HL‐60 cells. Induction of differentiation of the cells towards either granulocytes or macrophages resulted in a profound decrease (> 95%) in the concentration of MPO mRNA levels, showing that gene expression of MPO mRNA is closely linked to the stage of development of myeloid cells. Studies using normal and leukemic hematopoietic cells confirmed these findings and showed that myeloblasts and promyelocytes contain MPO mRNA. Rate of transcription of MPO was measured by a nuclear run‐on assay in wild‐type and day 3– and day –4 differentiated HL‐60 cells and was nearly the same in all three. In contrast, rate of transcription of c‐myc in the same nuclei became almost undetectable with induction of differentiation. Overall transcription decreased by 60% and 80% on day 3 and 4 of differentiation, respectively, compared to wild‐type cells. Stability of mature MPO mRNA was also measured and found to be the same in wild‐type and differentiated HL‐60. Half‐life of MPO hnRNA was ≤ 30 min in wild‐type HL‐60; nevertheless, this hnRNA was easily detectable 3 days after induction of differentiation of these cells. Taken together, the results show that decreased expression of MPO mRNA with differentiation occurs in part post‐transcriptionally, possibly due to a failure in RNA processing. In addition, as overall transcription decreases during differentiation, MPO transcription is concomitantly reduced. This indicates that transcriptional and post‐transcriptional mechanisms cooperate in the control of MPO gene expression.

Original languageEnglish (US)
Pages (from-to)215-225
Number of pages11
JournalJournal of Cellular Physiology
Volume136
Issue number2
DOIs
StatePublished - Aug 1988

Fingerprint

Gene Expression Regulation
Gene expression
Peroxidase
Transcription
Messenger RNA
HL-60 Cells
Granulocyte Precursor Cells
Cells
Cell Differentiation
Gene Expression
Nuclear RNA
Macrophages
Myeloid Cells
Heme
Granulocytes
Introns
Half-Life
Assays
Leukemia
Neutrophils

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Regulation of gene expression of myeloperoxidase during myeloid differentiation. / Tobler, Andreas; Miller, Carl W.; Johnson, Keith R.; Selsted, Michael E.; Rovera, Giovanni; Koeffler, H. Phillip.

In: Journal of Cellular Physiology, Vol. 136, No. 2, 08.1988, p. 215-225.

Research output: Contribution to journalArticle

Tobler, Andreas ; Miller, Carl W. ; Johnson, Keith R. ; Selsted, Michael E. ; Rovera, Giovanni ; Koeffler, H. Phillip. / Regulation of gene expression of myeloperoxidase during myeloid differentiation. In: Journal of Cellular Physiology. 1988 ; Vol. 136, No. 2. pp. 215-225.
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abstract = "Myeloperoxidase (MPO) is a major heme enzyme involved in inflammatory responses of polymorphonuclear leukocytes. Using cDNA and intron specific probes for MPO we studied the regulation of MPO expression during myeloid differentiation of the promyelocytic HL‐60 leukemia cell line. Mature MPO mRNA species of 3.3, 2.8 and 1.6 kb and heterogenous nuclear (hn)RNA of > 8 and ∼4 kb were observed in wildtype HL‐60 cells. Induction of differentiation of the cells towards either granulocytes or macrophages resulted in a profound decrease (> 95{\%}) in the concentration of MPO mRNA levels, showing that gene expression of MPO mRNA is closely linked to the stage of development of myeloid cells. Studies using normal and leukemic hematopoietic cells confirmed these findings and showed that myeloblasts and promyelocytes contain MPO mRNA. Rate of transcription of MPO was measured by a nuclear run‐on assay in wild‐type and day 3– and day –4 differentiated HL‐60 cells and was nearly the same in all three. In contrast, rate of transcription of c‐myc in the same nuclei became almost undetectable with induction of differentiation. Overall transcription decreased by 60{\%} and 80{\%} on day 3 and 4 of differentiation, respectively, compared to wild‐type cells. Stability of mature MPO mRNA was also measured and found to be the same in wild‐type and differentiated HL‐60. Half‐life of MPO hnRNA was ≤ 30 min in wild‐type HL‐60; nevertheless, this hnRNA was easily detectable 3 days after induction of differentiation of these cells. Taken together, the results show that decreased expression of MPO mRNA with differentiation occurs in part post‐transcriptionally, possibly due to a failure in RNA processing. In addition, as overall transcription decreases during differentiation, MPO transcription is concomitantly reduced. This indicates that transcriptional and post‐transcriptional mechanisms cooperate in the control of MPO gene expression.",
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