Regulation of Connexin32 by ephrin receptors and T-cell protein-tyrosine phosphatase

Andrew J. Trease, Hanjun Li, Gaelle Spagnol, Li Zheng, Kelly L. Stauch, Paul L Sorgen

Research output: Contribution to journalArticle

Abstract

Gap junctions are intercellular conduits that permit the passage of ions, small metabolites, and signaling molecules between cells. Connexin32 (Cx32) is a major gap junction protein in the liver and brain. Phosphorylation is integral to regulating connexin assembly, degradation, and electrical and metabolic coupling, as well as to interactions with molecular partners. Cx32 contains two intracellular tyrosine residues, and tyrosine phosphorylation of Cx32 has been detected after activation of the epidermal growth factor receptor; however, the specific tyrosine residue and the functional implication of this phosphorylation remain unknown. To address the limited available information on Cx32 regulation by tyrosine kinases, here we used the Cx32 C-terminal (CT) domain in an in vitro kinase-screening assay, which identified ephrin (Eph) receptor family members as tyrosine kinases that phosphorylate Cx32. We found that EphB1 and EphA1 phosphorylate the Cx32CT domain residue Tyr243. Unlike for Cx43, the tyrosine phosphorylation of the Cx32CT increased gap junction intercellular communication. We also demonstrated that T-cell protein-tyrosine phosphatase dephosphorylates pTyr243. The data presented above along with additional examples throughout the literature of gap junction regulation by kinases, indicate that one cannot extrapolate the effect of a kinase on one connexin to another.

Original languageEnglish (US)
Pages (from-to)341-350
Number of pages10
JournalJournal of Biological Chemistry
Volume294
Issue number1
DOIs
StatePublished - Jan 1 2019

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Non-Receptor Type 2 Protein Tyrosine Phosphatase
Eph Family Receptors
Phosphorylation
Connexins
Tyrosine
Gap Junctions
Phosphotransferases
Protein-Tyrosine Kinases
Connexin 43
Metabolites
Epidermal Growth Factor Receptor
Liver
Assays
Brain
Screening
Chemical activation
Ions
Degradation
Molecules
Communication

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Regulation of Connexin32 by ephrin receptors and T-cell protein-tyrosine phosphatase. / Trease, Andrew J.; Li, Hanjun; Spagnol, Gaelle; Zheng, Li; Stauch, Kelly L.; Sorgen, Paul L.

In: Journal of Biological Chemistry, Vol. 294, No. 1, 01.01.2019, p. 341-350.

Research output: Contribution to journalArticle

Trease, Andrew J. ; Li, Hanjun ; Spagnol, Gaelle ; Zheng, Li ; Stauch, Kelly L. ; Sorgen, Paul L. / Regulation of Connexin32 by ephrin receptors and T-cell protein-tyrosine phosphatase. In: Journal of Biological Chemistry. 2019 ; Vol. 294, No. 1. pp. 341-350.
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