Regulation of B cell tolerance by murine gangliosides

Harold C. Miller, William G. Chaney, Norman R. Klinman, Walter J. Esselman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The potential role of gangliosides as modulators of the triggering of neonatal primary B lymphocytes at the single precursor cell level was evaluated. Tolerance was induced in splenic fragment cultures containing an excess of carrier-primed T cells. Gangliosides at low concentrations (20 ng/culture) abrogated the tolerogenic effect of haptens presented on carriers not recognized by environmental T cells. The permanent arrest of immature B-cell responsiveness resulting from tolerogen treatment was eliminated by the presence of gangliosides during tolerogen treatment. The active moiety in the glycolipid preparation which protected B cells during tolerogen treatment was separated by ion-exchange chromatography and demonstrated to be in the disialoganglioside fraction.

Original languageEnglish (US)
Pages (from-to)390-395
Number of pages6
JournalCellular Immunology
Volume67
Issue number2
DOIs
StatePublished - Mar 1 1982

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Gangliosides
B-Lymphocytes
T-Lymphocytes
B-Lymphoid Precursor Cells
Haptens
Glycolipids
Ion Exchange Chromatography
Tolerogen

ASJC Scopus subject areas

  • Immunology

Cite this

Miller, H. C., Chaney, W. G., Klinman, N. R., & Esselman, W. J. (1982). Regulation of B cell tolerance by murine gangliosides. Cellular Immunology, 67(2), 390-395. https://doi.org/10.1016/0008-8749(82)90230-1

Regulation of B cell tolerance by murine gangliosides. / Miller, Harold C.; Chaney, William G.; Klinman, Norman R.; Esselman, Walter J.

In: Cellular Immunology, Vol. 67, No. 2, 01.03.1982, p. 390-395.

Research output: Contribution to journalArticle

Miller, HC, Chaney, WG, Klinman, NR & Esselman, WJ 1982, 'Regulation of B cell tolerance by murine gangliosides', Cellular Immunology, vol. 67, no. 2, pp. 390-395. https://doi.org/10.1016/0008-8749(82)90230-1
Miller, Harold C. ; Chaney, William G. ; Klinman, Norman R. ; Esselman, Walter J. / Regulation of B cell tolerance by murine gangliosides. In: Cellular Immunology. 1982 ; Vol. 67, No. 2. pp. 390-395.
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