Regulation and expression of transforming growth factor type-ß during early mammalian development

David Lee Kelly, Wendy J. Campbell, Jay Tiesman, A Angie Rizzino

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have examined the effect of differentiation on the expression of different members of the transforming growth factor type-beta (TGF-β) family using embryonal carcinoma (EC) cells and early mammalian embryos. We determined that TGF-β activity increases approximately 25-100% when the mouse EC cell line, F9, is induced to differentiate with retinoic acid (RA). Interestingly, the increased TGF-β activity reflects the induction of TGF-β2 secretion following differentiation of both F9 EC cells and the human EC cell line, NT2/D1. Using the technique of reverse transcription-polymerase chain reaction (RT-PCR), we have verified that differentiation induces the expression of TGF-β2 as well as a distant member of the TGF-β family, Vgr-1. Transcripts for TGF-β2 and Vgr-1 were readily detected in the differentiated cells of F9 and PC-13 but not in their undifferentiated counterparts. Moreover, TGF-β2 mRNA was readily detected in NT2/D1 cells following differentiation. In addition, transcripts for TGF-β2 were detected by RT-PCR in mouse morulae, preimplantation blastocysts and cultured blastocysts. Based on the data presented, it appears that the expression of both TGF-β2 and Vgr-1 is closely associated with the induction of differentiation during early development.

Original languageEnglish (US)
Pages (from-to)227-242
Number of pages16
JournalCytotechnology
Volume4
Issue number3
DOIs
StatePublished - Nov 1 1990

Fingerprint

Transforming Growth Factors
Transforming Growth Factor beta
Embryonal Carcinoma Stem Cells
Polymerase chain reaction
Cells
Blastocyst
Transcription
Reverse Transcription
Intercellular Signaling Peptides and Proteins
Mammalian Embryo
Morula
Cell Line
Polymerase Chain Reaction
Tretinoin
Cell Differentiation
Messenger RNA
Acids

Keywords

  • embryonal carcinoma cells
  • mammalian embryogenesis
  • polymerase chain reaction
  • transforming growth factor type-beta

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

Cite this

Regulation and expression of transforming growth factor type-ß during early mammalian development. / Kelly, David Lee; Campbell, Wendy J.; Tiesman, Jay; Rizzino, A Angie.

In: Cytotechnology, Vol. 4, No. 3, 01.11.1990, p. 227-242.

Research output: Contribution to journalArticle

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