Regulation and characterization of the interferon-α present in patients with advanced human immunodeficiency virus type 1 disease

Susan Swindells, Tonia Baldwin, Colleen Kelly, Lisa Baca-Regen, Larry Loomis, Diane Post, Beda Brichacek, Mario Stevenson, Edward A. Dominguez, Ramakrishna Reddy, Rose Klein, Mei June Liao, Douglas Testa, Thomas L McDonald, Joseph Bellanti, Simon Skurkovich, Howard Eliot Gendelman

Research output: Contribution to journalArticle

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Abstract

To examine a possible association between plasma viremia and interferon- α (IFN-α) in patients with the acquired immunodeficiency syndrome (AIDS), we performed IFN plasma immunoadsorption by apheresis (IFN-α apheresis) in four volunteers with AIDS who had sustained levels of endogenous plasma IFN- α. IFN-α apheresis with two plasma volume exchanges was performed daily for 5 days. Clinical signs and symptoms and hematologic, virologic, and immunologic parameters were monitored. Two subjects developed anemia from phlebotomy, and one had a catheter-associated bacteremia. The IFN-α apheresis was effective only in transiently removing IFN-α: depletion of IFN-α led only to its rapid reconstitution. Cell-associated HIV-1 was unchanged, but three of four subjects had a modest decrease in culturable plasma virus burden following the procedures. The recovery of in vivo HIV-1- related IFN-α by apheresis allowed its biologic and biochemical characterization. The HIV-1 IFN-α showed characteristics on ELISA, western blot, and biologic assays similar to two subspecies of the natural protein. The natural, recombinant, and HIV-1-induced IFN-αs demonstrated nearly identical antiviral activities. The HIV-1 IFN-α eluted from the column was not acid labile. The inability of large amounts of plasma IFN-α found in some patients with AIDS to affect viral burden likely reflects properties of the virus or of host factors independent of IFN-α.

Original languageEnglish (US)
Pages (from-to)127-137
Number of pages11
JournalJournal of Interferon and Cytokine Research
Volume16
Issue number2
DOIs
StatePublished - Feb 1996

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Virus Diseases
Interferons
HIV-1
Blood Component Removal
Acquired Immunodeficiency Syndrome
Viruses
Plasma Exchange
Phlebotomy
Plasma Volume
Viremia
Bacteremia
Viral Load
Biological Assay
Signs and Symptoms
Antiviral Agents
Anemia
Volunteers
Catheters
Western Blotting
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

Cite this

Regulation and characterization of the interferon-α present in patients with advanced human immunodeficiency virus type 1 disease. / Swindells, Susan; Baldwin, Tonia; Kelly, Colleen; Baca-Regen, Lisa; Loomis, Larry; Post, Diane; Brichacek, Beda; Stevenson, Mario; Dominguez, Edward A.; Reddy, Ramakrishna; Klein, Rose; Liao, Mei June; Testa, Douglas; McDonald, Thomas L; Bellanti, Joseph; Skurkovich, Simon; Gendelman, Howard Eliot.

In: Journal of Interferon and Cytokine Research, Vol. 16, No. 2, 02.1996, p. 127-137.

Research output: Contribution to journalArticle

Swindells, S, Baldwin, T, Kelly, C, Baca-Regen, L, Loomis, L, Post, D, Brichacek, B, Stevenson, M, Dominguez, EA, Reddy, R, Klein, R, Liao, MJ, Testa, D, McDonald, TL, Bellanti, J, Skurkovich, S & Gendelman, HE 1996, 'Regulation and characterization of the interferon-α present in patients with advanced human immunodeficiency virus type 1 disease', Journal of Interferon and Cytokine Research, vol. 16, no. 2, pp. 127-137. https://doi.org/10.1089/jir.1996.16.127
Swindells, Susan ; Baldwin, Tonia ; Kelly, Colleen ; Baca-Regen, Lisa ; Loomis, Larry ; Post, Diane ; Brichacek, Beda ; Stevenson, Mario ; Dominguez, Edward A. ; Reddy, Ramakrishna ; Klein, Rose ; Liao, Mei June ; Testa, Douglas ; McDonald, Thomas L ; Bellanti, Joseph ; Skurkovich, Simon ; Gendelman, Howard Eliot. / Regulation and characterization of the interferon-α present in patients with advanced human immunodeficiency virus type 1 disease. In: Journal of Interferon and Cytokine Research. 1996 ; Vol. 16, No. 2. pp. 127-137.
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