Reduced nuclear import of human immunodeficiency virus type 1 preintegration complexes in the presence of a prototypic nuclear targeting signal

J. Gulizia, M. P. Dempsey, N. Sharova, M. I. Bukrinsky, L. Spitz, D. Goldfarb, M. Stevenson

Research output: Contribution to journalComment/debate

40 Citations (Scopus)

Abstract

Nuclear import of the retroviral preintegration complex and integration of retroviral with host cell DNA are essential steps for completion of the virus life cycle. The preintegration complex of the lentivirus human immunodeficiency virus type 1 (HIV-1) displays karyophilic properties and, as a consequence, is rapidly directed to the host cell nucleus by an energy- dependent transport pathway. The karyophilic properties of nuclear proteins are governed by a nuclear localization sequence, the targeting function of which can be inhibited in the presence of excess targeting signals. Here we present evidence that the nuclear import of a large karyophile-the preintegration complex of HIV-1-is inhibited in the presence of a prototypic nuclear targeting signal of simian virus 40 T antigen. This points to a novel strategy which prevents establishment of the provirus by interrupting nuclear localization of HIV-1 DNA.

Original languageEnglish (US)
Pages (from-to)2021-2025
Number of pages5
JournalJournal of virology
Volume68
Issue number3
StatePublished - Mar 1 1994

Fingerprint

Cell Nucleus Active Transport
Human immunodeficiency virus 1
imports
HIV-1
Simian virus 40
proviruses
Proviruses
nuclear localization signals
Lentivirus
nuclear proteins
Viral Tumor Antigens
DNA
cell nucleus
Nuclear Proteins
Life Cycle Stages
Cell Nucleus
Viruses
antigens
viruses
energy

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Gulizia, J., Dempsey, M. P., Sharova, N., Bukrinsky, M. I., Spitz, L., Goldfarb, D., & Stevenson, M. (1994). Reduced nuclear import of human immunodeficiency virus type 1 preintegration complexes in the presence of a prototypic nuclear targeting signal. Journal of virology, 68(3), 2021-2025.

Reduced nuclear import of human immunodeficiency virus type 1 preintegration complexes in the presence of a prototypic nuclear targeting signal. / Gulizia, J.; Dempsey, M. P.; Sharova, N.; Bukrinsky, M. I.; Spitz, L.; Goldfarb, D.; Stevenson, M.

In: Journal of virology, Vol. 68, No. 3, 01.03.1994, p. 2021-2025.

Research output: Contribution to journalComment/debate

Gulizia, J, Dempsey, MP, Sharova, N, Bukrinsky, MI, Spitz, L, Goldfarb, D & Stevenson, M 1994, 'Reduced nuclear import of human immunodeficiency virus type 1 preintegration complexes in the presence of a prototypic nuclear targeting signal', Journal of virology, vol. 68, no. 3, pp. 2021-2025.
Gulizia, J. ; Dempsey, M. P. ; Sharova, N. ; Bukrinsky, M. I. ; Spitz, L. ; Goldfarb, D. ; Stevenson, M. / Reduced nuclear import of human immunodeficiency virus type 1 preintegration complexes in the presence of a prototypic nuclear targeting signal. In: Journal of virology. 1994 ; Vol. 68, No. 3. pp. 2021-2025.
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