Reduced heme oxygenase-1 expression in steatotic livers infected with hepatitis C virus

Maher Y. Abdalla, Meleah M. Mathahs, Iman M. Ahmad

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Hepatic nonalcoholic fatty liver disease (NAFLD) is known to exacerbate liver injury due to chronic hepatitis C infection. Heme oxygenase-1 (HO-1) is an important protective antioxidative defense enzyme that is known to be induced in response to NAFLD and other liver injuries. The aim of this study was to evaluate HO-1 expression in HCV infected human livers with concomitant NAFLD. Methods: We compared levels of HO-1 in NAFLD liver biopsies from patients with or without chronic HCV infection using immunohistochemistry, immunoblots and real time RT-PCR. We also evaluated frozen sections of liver with dihydroethidium (DHE) or dichlorofluorescein (DCF) fluorescence staining to evaluate O2- and peroxide production respectively. Results: HO-1 expression was only increased in NAFLD livers without HCV infection, while HCV infected livers showed reduced HO-1 levels, regardless whether NAFLD was present. In uninfected livers with NAFLD, HO-1 expression was primarily localized in hepatocytes containing fat and areas of injury around the central vein. However, both NAFLD with and without concomitant HCV infection showed high levels of O2- or peroxide production compared to normal human liver control samples. Conclusions: These findings support the hypothesis that NAFLD is an important process for hepatocyte oxidative stress and injury in liver diseases. They also suggest that HCV can repress HO-1 induction in vivo even when other inducers of HO-1 are present.

Original languageEnglish (US)
Pages (from-to)649-655
Number of pages7
JournalEuropean Journal of Internal Medicine
Volume23
Issue number7
DOIs
StatePublished - Oct 1 2012

Fingerprint

Heme Oxygenase-1
Hepacivirus
Liver
Peroxides
Wounds and Injuries
Infection
Hepatocytes
Non-alcoholic Fatty Liver Disease
Frozen Sections
Chronic Hepatitis C
Liver Diseases
Real-Time Polymerase Chain Reaction
Veins
Oxidative Stress
Fluorescence
Immunohistochemistry
Fats
Staining and Labeling
Biopsy

Keywords

  • HCV
  • HO-1
  • NAFLD
  • Oxidative stress

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Reduced heme oxygenase-1 expression in steatotic livers infected with hepatitis C virus. / Abdalla, Maher Y.; Mathahs, Meleah M.; Ahmad, Iman M.

In: European Journal of Internal Medicine, Vol. 23, No. 7, 01.10.2012, p. 649-655.

Research output: Contribution to journalArticle

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N2 - Hepatic nonalcoholic fatty liver disease (NAFLD) is known to exacerbate liver injury due to chronic hepatitis C infection. Heme oxygenase-1 (HO-1) is an important protective antioxidative defense enzyme that is known to be induced in response to NAFLD and other liver injuries. The aim of this study was to evaluate HO-1 expression in HCV infected human livers with concomitant NAFLD. Methods: We compared levels of HO-1 in NAFLD liver biopsies from patients with or without chronic HCV infection using immunohistochemistry, immunoblots and real time RT-PCR. We also evaluated frozen sections of liver with dihydroethidium (DHE) or dichlorofluorescein (DCF) fluorescence staining to evaluate O2- and peroxide production respectively. Results: HO-1 expression was only increased in NAFLD livers without HCV infection, while HCV infected livers showed reduced HO-1 levels, regardless whether NAFLD was present. In uninfected livers with NAFLD, HO-1 expression was primarily localized in hepatocytes containing fat and areas of injury around the central vein. However, both NAFLD with and without concomitant HCV infection showed high levels of O2- or peroxide production compared to normal human liver control samples. Conclusions: These findings support the hypothesis that NAFLD is an important process for hepatocyte oxidative stress and injury in liver diseases. They also suggest that HCV can repress HO-1 induction in vivo even when other inducers of HO-1 are present.

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