Reduced hamster usage and stress in propagating leishmania chagasi promastigotes using cryopreservation and saphenous vein inoculation

Soi Meng Lei, Amanda E. Ramer-Tait, Rebecca R. Dahlin-Laborde, Kathleen Mullin, Jeffrey K. Beetham

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, because L. chagasi parasites newly isolated from an infected hamster can be grown in culture for only several weeks before loss of function/phenotype occurs. In an effort to decrease animal usage and animal stress, experiments were performed to assess a more gentle inoculation procedure (saphenous vein inoculation) and the use of cryopreserved parasite cells for research experiments. Of 81 hamsters inoculated by the saphenous vein, 80 became infected as determined ante mortem, by display of clinical symptoms of leishmaniasis (onset of symptoms at 105 ± 22 days post-inoculation), and postmortem by the presence of parasites within the spleen. Splenic parasite load calculated for a subset (n 34) of infected hamsters was 124 to 26,177 Leishmania donovani infection units. Cryopreserved, and never-stored, cells were equivalent in all properties evaluated, including developmental changes in morphology during culture, culture growth rates, parasite resistance to serum-mediated lysis, and expression of developmentally regulated surface proteins major surface protease and promastigote surface antigen.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalJournal of Parasitology
Volume96
Issue number1
DOIs
StatePublished - Feb 1 2010

Fingerprint

saphenous vein
Leishmania infantum
cryopreservation
promastigotes
Leishmania donovani
Cryopreservation
Saphenous Vein
hamsters
Cricetinae
inoculation
vaccination
animal
leishmaniasis
Parasites
parasite
animal stress
parasites
signs and symptoms (animals and humans)
parasite resistance
parasite intensity

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Reduced hamster usage and stress in propagating leishmania chagasi promastigotes using cryopreservation and saphenous vein inoculation. / Lei, Soi Meng; Ramer-Tait, Amanda E.; Dahlin-Laborde, Rebecca R.; Mullin, Kathleen; Beetham, Jeffrey K.

In: Journal of Parasitology, Vol. 96, No. 1, 01.02.2010, p. 103-108.

Research output: Contribution to journalArticle

Lei, Soi Meng ; Ramer-Tait, Amanda E. ; Dahlin-Laborde, Rebecca R. ; Mullin, Kathleen ; Beetham, Jeffrey K. / Reduced hamster usage and stress in propagating leishmania chagasi promastigotes using cryopreservation and saphenous vein inoculation. In: Journal of Parasitology. 2010 ; Vol. 96, No. 1. pp. 103-108.
@article{4620fb6707f6457f9a31305cc27bbd01,
title = "Reduced hamster usage and stress in propagating leishmania chagasi promastigotes using cryopreservation and saphenous vein inoculation",
abstract = "Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, because L. chagasi parasites newly isolated from an infected hamster can be grown in culture for only several weeks before loss of function/phenotype occurs. In an effort to decrease animal usage and animal stress, experiments were performed to assess a more gentle inoculation procedure (saphenous vein inoculation) and the use of cryopreserved parasite cells for research experiments. Of 81 hamsters inoculated by the saphenous vein, 80 became infected as determined ante mortem, by display of clinical symptoms of leishmaniasis (onset of symptoms at 105 ± 22 days post-inoculation), and postmortem by the presence of parasites within the spleen. Splenic parasite load calculated for a subset (n 34) of infected hamsters was 124 to 26,177 Leishmania donovani infection units. Cryopreserved, and never-stored, cells were equivalent in all properties evaluated, including developmental changes in morphology during culture, culture growth rates, parasite resistance to serum-mediated lysis, and expression of developmentally regulated surface proteins major surface protease and promastigote surface antigen.",
author = "Lei, {Soi Meng} and Ramer-Tait, {Amanda E.} and Dahlin-Laborde, {Rebecca R.} and Kathleen Mullin and Beetham, {Jeffrey K.}",
year = "2010",
month = "2",
day = "1",
doi = "10.1645/GE-2192.1",
language = "English (US)",
volume = "96",
pages = "103--108",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "1",

}

TY - JOUR

T1 - Reduced hamster usage and stress in propagating leishmania chagasi promastigotes using cryopreservation and saphenous vein inoculation

AU - Lei, Soi Meng

AU - Ramer-Tait, Amanda E.

AU - Dahlin-Laborde, Rebecca R.

AU - Mullin, Kathleen

AU - Beetham, Jeffrey K.

PY - 2010/2/1

Y1 - 2010/2/1

N2 - Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, because L. chagasi parasites newly isolated from an infected hamster can be grown in culture for only several weeks before loss of function/phenotype occurs. In an effort to decrease animal usage and animal stress, experiments were performed to assess a more gentle inoculation procedure (saphenous vein inoculation) and the use of cryopreserved parasite cells for research experiments. Of 81 hamsters inoculated by the saphenous vein, 80 became infected as determined ante mortem, by display of clinical symptoms of leishmaniasis (onset of symptoms at 105 ± 22 days post-inoculation), and postmortem by the presence of parasites within the spleen. Splenic parasite load calculated for a subset (n 34) of infected hamsters was 124 to 26,177 Leishmania donovani infection units. Cryopreserved, and never-stored, cells were equivalent in all properties evaluated, including developmental changes in morphology during culture, culture growth rates, parasite resistance to serum-mediated lysis, and expression of developmentally regulated surface proteins major surface protease and promastigote surface antigen.

AB - Leishmania chagasi, a causal agent of visceral leishmaniasis, requires passage through lab animals such as hamsters to maintain its virulence. Hamster infection is typically accomplished via cardiac puncture or intraperitoneal injection, procedures accompanied by risks of increased animal stress and death. The use of the hamster model also necessitates a regular supply of infected animals, because L. chagasi parasites newly isolated from an infected hamster can be grown in culture for only several weeks before loss of function/phenotype occurs. In an effort to decrease animal usage and animal stress, experiments were performed to assess a more gentle inoculation procedure (saphenous vein inoculation) and the use of cryopreserved parasite cells for research experiments. Of 81 hamsters inoculated by the saphenous vein, 80 became infected as determined ante mortem, by display of clinical symptoms of leishmaniasis (onset of symptoms at 105 ± 22 days post-inoculation), and postmortem by the presence of parasites within the spleen. Splenic parasite load calculated for a subset (n 34) of infected hamsters was 124 to 26,177 Leishmania donovani infection units. Cryopreserved, and never-stored, cells were equivalent in all properties evaluated, including developmental changes in morphology during culture, culture growth rates, parasite resistance to serum-mediated lysis, and expression of developmentally regulated surface proteins major surface protease and promastigote surface antigen.

UR - http://www.scopus.com/inward/record.url?scp=77950383006&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950383006&partnerID=8YFLogxK

U2 - 10.1645/GE-2192.1

DO - 10.1645/GE-2192.1

M3 - Article

C2 - 19835434

AN - SCOPUS:77950383006

VL - 96

SP - 103

EP - 108

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 1

ER -