Reduced genomic potential for secreted plant cell-wall-degrading enzymes in the ectomycorrhizal fungus Amanita bisporigera, based on the secretome of Trichoderma reesei

Subashini Nagendran, Heather E Hallen-Adams, Janet M. Paper, Nighat Aslam, Jonathan D. Walton

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Based on the analysis of its genome sequence, the ectomycorrhizal (ECM) basidiomycetous fungus Laccaria bicolor was shown to be lacking many of the major classes of secreted enzymes that depolymerize plant cell wall polysaccharides. To test whether this is also a feature of other ECM fungi, we searched a survey genome database of Amanita bisporigera with the proteins found in the secretome of Trichoderma reesei (syn. Hypocrea jecorina), a biochemically well-characterized industrial fungus. Additional proteins were also used as queries to compensate for major groups of cell-wall-degrading enzymes lacking in the secretome of T. reesei and to substantiate conclusions drawn from the T. reesei collection. By MS/MS-based "shotgun" proteomics, 80 proteins were identified in culture filtrates of T. reesei strain RUTC30 grown on corn cell walls and in a commercial "cellulase" preparation, Spezyme CP. The two T. reesei enzyme preparations were qualitatively and quantitatively similar, the most striking difference being the lack of at least five major peptidases from the commercial enzyme mixture. Based on our analysis of A. bisporigera, this ECM fungus is deficient in many major classes of cell-wall-degrading enzymes, including both glycosyl hydrolases and carbohydrate esterases. By comparison, the genomes of the saprophytic basidiomycetes Coprinopsis cinerea and Galerina marginata (using a genome survey sequence approximately equivalent in depth to that of A. bisporigera) have, like T. reesei, a much more complete complement of cell-wall-degrading enzymes.

Original languageEnglish (US)
Pages (from-to)427-435
Number of pages9
JournalFungal Genetics and Biology
Volume46
Issue number5
DOIs
StatePublished - May 1 2009

Fingerprint

Amanita
Trichoderma
Plant Cells
Cell Wall
Fungi
Enzymes
Genome
Laccaria
Hypocrea
Basidiomycota
Proteins
Cellulase
Firearms
Hydrolases
Esterases
Proteomics
Zea mays
Polysaccharides
Peptide Hydrolases
Carbohydrates

Keywords

  • Cellulase
  • Proteomics
  • Xylanase

ASJC Scopus subject areas

  • Microbiology
  • Genetics

Cite this

Reduced genomic potential for secreted plant cell-wall-degrading enzymes in the ectomycorrhizal fungus Amanita bisporigera, based on the secretome of Trichoderma reesei. / Nagendran, Subashini; Hallen-Adams, Heather E; Paper, Janet M.; Aslam, Nighat; Walton, Jonathan D.

In: Fungal Genetics and Biology, Vol. 46, No. 5, 01.05.2009, p. 427-435.

Research output: Contribution to journalArticle

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