Reduced expression of mismatch repair genes in colorectal cancer patients in Egypt

Amr S Soliman, Melissa L. Bondy, Yongli Guan, Sami El-Badawi, Nadia Mokhtar, Sherif Bayomi, Ahmed A. Raouf, Sohair Ismail, R. Sue McPherson, Tarek F. Abdel-Hakim, R. Palmer Beasley, Bernard Levin, Qingyi Wei

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

An Egyptian hospital-based pilot case-control study was conducted to investigate the relationship between the expression level of mismatch repair (MMR) genes and the risk of colorectal cancer. The relative expression of five known MMR genes, i.e., hMSH2, hMLH1, hPMS1, hPMS2, and GTBP/hMSH6, was measured by a multiplex reverse transcriptase (RT)-polymerase chain reaction (PCR) in peripheral blood lymphocytes from 31 colorectal cancer patients and 47 age- and-sex matched controls. The expression of hMSH2, GTBP/hMSH6, hPMS1 and hPMS2 tended to be lower in patients than controls, but only the difference in hPMS2 expression was statistically significant (p<0.01). Although 50% of the cases had chemotherapy or radiotherapy within the last Six months before the blood was drawn, their gene expression was not statistically different from those who had not Undergone such therapies. After adjustment for age and sex, the odds ratios (OR) calculated from a logistical regression model, using the median levels of gene expression of controls as cut-off values, indicated that increased risk was associated with reduced expressions of both hPMS1 (OR = 3.97, 95% confidence interval (CI) = 1.04 to 7.65) and hPMS2 (OR = 2.86, 95% CI = 1.05 to 7.76). Although the results of this study were inconclusive because of the small sample size and use of prevalent cases, it is biologically plausible that patients with colorectal cancers may have a lower expression of MMR genes than healthy controls because malfunction of these genes has been shown in hereditary nonpolyposis colon cancer. The involvement of low hPMS2 expression in colon cancer risk seems to be unique in the Egyptian population. Further studies with newly diagnosed patients before they begin therapy will provide more convincing data about the role of MMR gene expression in the etiology of colorectal cancers in Egypt.

Original languageEnglish (US)
Pages (from-to)1315-1319
Number of pages5
JournalInternational Journal of Oncology
Volume12
Issue number6
StatePublished - Jun 1 1998

Fingerprint

DNA Mismatch Repair
Egypt
Colorectal Neoplasms
Odds Ratio
Gene Expression
Genes
Confidence Intervals
Hereditary Nonpolyposis Colorectal Neoplasms
Multiplex Polymerase Chain Reaction
Sex Ratio
Reverse Transcriptase Polymerase Chain Reaction
Sample Size
Colonic Neoplasms
Case-Control Studies
Radiotherapy
Lymphocytes
Drug Therapy
Therapeutics
Population

Keywords

  • Colorectal cancer
  • Gene expression
  • Mismatch repair
  • Polymerase chain reaction
  • Reverse transcription

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Soliman, A. S., Bondy, M. L., Guan, Y., El-Badawi, S., Mokhtar, N., Bayomi, S., ... Wei, Q. (1998). Reduced expression of mismatch repair genes in colorectal cancer patients in Egypt. International Journal of Oncology, 12(6), 1315-1319.

Reduced expression of mismatch repair genes in colorectal cancer patients in Egypt. / Soliman, Amr S; Bondy, Melissa L.; Guan, Yongli; El-Badawi, Sami; Mokhtar, Nadia; Bayomi, Sherif; Raouf, Ahmed A.; Ismail, Sohair; McPherson, R. Sue; Abdel-Hakim, Tarek F.; Beasley, R. Palmer; Levin, Bernard; Wei, Qingyi.

In: International Journal of Oncology, Vol. 12, No. 6, 01.06.1998, p. 1315-1319.

Research output: Contribution to journalArticle

Soliman, AS, Bondy, ML, Guan, Y, El-Badawi, S, Mokhtar, N, Bayomi, S, Raouf, AA, Ismail, S, McPherson, RS, Abdel-Hakim, TF, Beasley, RP, Levin, B & Wei, Q 1998, 'Reduced expression of mismatch repair genes in colorectal cancer patients in Egypt', International Journal of Oncology, vol. 12, no. 6, pp. 1315-1319.
Soliman AS, Bondy ML, Guan Y, El-Badawi S, Mokhtar N, Bayomi S et al. Reduced expression of mismatch repair genes in colorectal cancer patients in Egypt. International Journal of Oncology. 1998 Jun 1;12(6):1315-1319.
Soliman, Amr S ; Bondy, Melissa L. ; Guan, Yongli ; El-Badawi, Sami ; Mokhtar, Nadia ; Bayomi, Sherif ; Raouf, Ahmed A. ; Ismail, Sohair ; McPherson, R. Sue ; Abdel-Hakim, Tarek F. ; Beasley, R. Palmer ; Levin, Bernard ; Wei, Qingyi. / Reduced expression of mismatch repair genes in colorectal cancer patients in Egypt. In: International Journal of Oncology. 1998 ; Vol. 12, No. 6. pp. 1315-1319.
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