Reduced adenosine receptor regulation of TNF-α in peripheral blood mononuclear cells from multiple sclerosis patients

M. Mayne, Jonathan Geiger, C. Power

Research output: Contribution to journalArticle

Abstract

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine involved in multiple sclerosis (MS) pathogenesis. Adenosine, working through specific subtypes of cell surface adenosine receptors can inhibit TNF-α release. In this study, we determined the extent to which activation of adenosine A1 receptors reduced the secretion of TNF-α from U937 cells and peripheral blood mononuclear cells (PBMCs) from control (n=8) and MS patients (n=8). U937 cells and PBMCs were stimulated with a phorbol-12-myristate-13-acetate (PMA) and phytohemagglutinin (PHA) (5 ng/10μg/ml) for 4 h. and TNF-α levels were measured by ELISA. In U937 cells, the adenosine A1 receptor agonist R-phenylisopropyladenosine (RPIA) dose-dependently reduced TNF-α levels (IC50 100 nM) by up to 78±9% at 1μM; the reduction was blocked with the A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX). RPIA significantly (p<0.007) reduced TNF-α levels by 50±8% in PBMCs from controls but not in PBMCs from MS patients (13±9%). DPCPX blocked the RPIA-mediated inhibition of PBMC TNF-α synthesis. The diminished inhibition of TNF-α release by RPIA in PBMCs from MS patients was not due to reduced A1 receptor levels since immunoblots showed no differences between control and MS PBMCs samples. Desensitization of adenosine A1 receptors in PBMCs of MS patients may lead to higher serum levels of TNF-α and increased inflammatory responses.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

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Purinergic P1 Receptors
Multiple Sclerosis
Blood Cells
Blood
Tumor Necrosis Factor-alpha
Phenylisopropyladenosine
U937 Cells
Adenosine A1 Receptors
Adenosine A1 Receptor Agonists
Cell Surface Receptors
Phytohemagglutinins
Adenosine
Inhibitory Concentration 50
Acetates
Chemical activation
Enzyme-Linked Immunosorbent Assay
Cells
Cytokines

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Reduced adenosine receptor regulation of TNF-α in peripheral blood mononuclear cells from multiple sclerosis patients. / Mayne, M.; Geiger, Jonathan; Power, C.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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abstract = "Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine involved in multiple sclerosis (MS) pathogenesis. Adenosine, working through specific subtypes of cell surface adenosine receptors can inhibit TNF-α release. In this study, we determined the extent to which activation of adenosine A1 receptors reduced the secretion of TNF-α from U937 cells and peripheral blood mononuclear cells (PBMCs) from control (n=8) and MS patients (n=8). U937 cells and PBMCs were stimulated with a phorbol-12-myristate-13-acetate (PMA) and phytohemagglutinin (PHA) (5 ng/10μg/ml) for 4 h. and TNF-α levels were measured by ELISA. In U937 cells, the adenosine A1 receptor agonist R-phenylisopropyladenosine (RPIA) dose-dependently reduced TNF-α levels (IC50 100 nM) by up to 78±9{\%} at 1μM; the reduction was blocked with the A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX). RPIA significantly (p<0.007) reduced TNF-α levels by 50±8{\%} in PBMCs from controls but not in PBMCs from MS patients (13±9{\%}). DPCPX blocked the RPIA-mediated inhibition of PBMC TNF-α synthesis. The diminished inhibition of TNF-α release by RPIA in PBMCs from MS patients was not due to reduced A1 receptor levels since immunoblots showed no differences between control and MS PBMCs samples. Desensitization of adenosine A1 receptors in PBMCs of MS patients may lead to higher serum levels of TNF-α and increased inflammatory responses.",
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