Redox status in workers occupationally exposed to long-term low levels of ionizing radiation: A pilot study

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Abstract

Objectives: Reactive oxygen species (ROS), including superoxide (O2•−), play an important role in the biological effects of ionizing radiation. The human body has developed different antioxidant systems to defend against excessive levels of ROS. The aim of the present study is to investigate the redox status changes in the blood of radiologic technologists and compare these changes to control individuals. Methods: We enrolled 60 medical workers: 20 occupationally exposed to ionizing radiation (all radiologic technologists), divided in three subgroups: conventional radiography (CR), computerized tomography (CT), and interventional radiography (IR) and 40 age- and gender-matched unexposed controls. Levels of O2•− and malondialdehyde (MDA) in blood were measured as an index of redox status, as were the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase. Redox status was also assessed by measuring levels of reduced and oxidized glutathione (GSH, GSSG, respectively). Results: Levels of O2•− and MDA, and SOD activity in the blood of IR and CT-exposed subjects were significantly higher than both the CR-exposed subjects and control individuals. However, there were no statistically significant differences in the levels of catalase, GSH and ratio of GSH/GSSG between exposed workers and control individuals. Discussion: This study suggests that healthcare workers in CT and IR occupationally exposed to radiation have an elevated circulating redox status as compared to unexposed individuals.

Original languageEnglish (US)
Pages (from-to)139-145
Number of pages7
JournalRedox Report
Volume21
Issue number3
DOIs
StatePublished - May 3 2016

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Interventional Radiography
Radiography
Ionizing radiation
Ionizing Radiation
Oxidation-Reduction
Glutathione Disulfide
Computerized tomography
Tomography
Malondialdehyde
Blood
Catalase
Superoxide Dismutase
Reactive Oxygen Species
Antioxidants
Human Body
Superoxides
Glutathione
Radiation
Delivery of Health Care
Enzymes

Keywords

  • Catalase
  • Glutathione
  • Low-level ionizing radiation
  • Malondialdehyde
  • Radiologic technologists
  • Superoxide
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical

Cite this

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title = "Redox status in workers occupationally exposed to long-term low levels of ionizing radiation: A pilot study",
abstract = "Objectives: Reactive oxygen species (ROS), including superoxide (O2•−), play an important role in the biological effects of ionizing radiation. The human body has developed different antioxidant systems to defend against excessive levels of ROS. The aim of the present study is to investigate the redox status changes in the blood of radiologic technologists and compare these changes to control individuals. Methods: We enrolled 60 medical workers: 20 occupationally exposed to ionizing radiation (all radiologic technologists), divided in three subgroups: conventional radiography (CR), computerized tomography (CT), and interventional radiography (IR) and 40 age- and gender-matched unexposed controls. Levels of O2•− and malondialdehyde (MDA) in blood were measured as an index of redox status, as were the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase. Redox status was also assessed by measuring levels of reduced and oxidized glutathione (GSH, GSSG, respectively). Results: Levels of O2•− and MDA, and SOD activity in the blood of IR and CT-exposed subjects were significantly higher than both the CR-exposed subjects and control individuals. However, there were no statistically significant differences in the levels of catalase, GSH and ratio of GSH/GSSG between exposed workers and control individuals. Discussion: This study suggests that healthcare workers in CT and IR occupationally exposed to radiation have an elevated circulating redox status as compared to unexposed individuals.",
keywords = "Catalase, Glutathione, Low-level ionizing radiation, Malondialdehyde, Radiologic technologists, Superoxide, Superoxide dismutase",
author = "Ahmad, {Iman M} and Temme, {James Bernard} and Abdalla, {Maher Y} and Zimmerman, {Matthew C}",
year = "2016",
month = "5",
day = "3",
doi = "10.1080/13510002.2015.1101891",
language = "English (US)",
volume = "21",
pages = "139--145",
journal = "Redox Report",
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number = "3",

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T1 - Redox status in workers occupationally exposed to long-term low levels of ionizing radiation

T2 - A pilot study

AU - Ahmad, Iman M

AU - Temme, James Bernard

AU - Abdalla, Maher Y

AU - Zimmerman, Matthew C

PY - 2016/5/3

Y1 - 2016/5/3

N2 - Objectives: Reactive oxygen species (ROS), including superoxide (O2•−), play an important role in the biological effects of ionizing radiation. The human body has developed different antioxidant systems to defend against excessive levels of ROS. The aim of the present study is to investigate the redox status changes in the blood of radiologic technologists and compare these changes to control individuals. Methods: We enrolled 60 medical workers: 20 occupationally exposed to ionizing radiation (all radiologic technologists), divided in three subgroups: conventional radiography (CR), computerized tomography (CT), and interventional radiography (IR) and 40 age- and gender-matched unexposed controls. Levels of O2•− and malondialdehyde (MDA) in blood were measured as an index of redox status, as were the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase. Redox status was also assessed by measuring levels of reduced and oxidized glutathione (GSH, GSSG, respectively). Results: Levels of O2•− and MDA, and SOD activity in the blood of IR and CT-exposed subjects were significantly higher than both the CR-exposed subjects and control individuals. However, there were no statistically significant differences in the levels of catalase, GSH and ratio of GSH/GSSG between exposed workers and control individuals. Discussion: This study suggests that healthcare workers in CT and IR occupationally exposed to radiation have an elevated circulating redox status as compared to unexposed individuals.

AB - Objectives: Reactive oxygen species (ROS), including superoxide (O2•−), play an important role in the biological effects of ionizing radiation. The human body has developed different antioxidant systems to defend against excessive levels of ROS. The aim of the present study is to investigate the redox status changes in the blood of radiologic technologists and compare these changes to control individuals. Methods: We enrolled 60 medical workers: 20 occupationally exposed to ionizing radiation (all radiologic technologists), divided in three subgroups: conventional radiography (CR), computerized tomography (CT), and interventional radiography (IR) and 40 age- and gender-matched unexposed controls. Levels of O2•− and malondialdehyde (MDA) in blood were measured as an index of redox status, as were the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase. Redox status was also assessed by measuring levels of reduced and oxidized glutathione (GSH, GSSG, respectively). Results: Levels of O2•− and MDA, and SOD activity in the blood of IR and CT-exposed subjects were significantly higher than both the CR-exposed subjects and control individuals. However, there were no statistically significant differences in the levels of catalase, GSH and ratio of GSH/GSSG between exposed workers and control individuals. Discussion: This study suggests that healthcare workers in CT and IR occupationally exposed to radiation have an elevated circulating redox status as compared to unexposed individuals.

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