Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics

Sophie Alvarez, Ashley Galant, Joseph M. Jez, Leslie M. Hicks

Research output: Contribution to journalArticle

Abstract

ROS, including hydrogen peroxide (H2O2), can serve as cellular signaling molecules following oxidative stress. Analysis of the redox state of proteins in Brassica juncea roots by 2-DE proteomics following treatment with either exogenous H2O2 or buthionine sulfoximine, which depletes glutathione to cause accumulation of endogenous H2O2, led to the identification of different sets of proteins. These data suggest that exogenous and endogenous oxidative stresses trigger specialized responses.

Original languageEnglish (US)
Pages (from-to)1346-1350
Number of pages5
JournalProteomics
Volume11
Issue number7
DOIs
StatePublished - Apr 1 2011

Fingerprint

Mustard Plant
Oxidative stress
Proteomics
Oxidation-Reduction
Oxidative Stress
Cell signaling
Buthionine Sulfoximine
Hydrogen Peroxide
Glutathione
Proteins
Molecules

Keywords

  • 2-DE
  • Buthionine sulfoximine
  • Hydrogen peroxide
  • Oxidative stress
  • Plant proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics. / Alvarez, Sophie; Galant, Ashley; Jez, Joseph M.; Hicks, Leslie M.

In: Proteomics, Vol. 11, No. 7, 01.04.2011, p. 1346-1350.

Research output: Contribution to journalArticle

Alvarez, Sophie ; Galant, Ashley ; Jez, Joseph M. ; Hicks, Leslie M. / Redox-regulatory mechanisms induced by oxidative stress in Brassica juncea roots monitored by 2-DE proteomics. In: Proteomics. 2011 ; Vol. 11, No. 7. pp. 1346-1350.
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