Red yeast rice fermentation with Bacillus subtilis B2 under blue light-emitting diodes increases antioxidant secondary products (Manuscript ID

BPBSE-18-0387)

Punniyakotti Elumalai, Yool Jin Park, Min Cho, Patrick J Shea, Byung Taek Oh

Research output: Contribution to journalArticle

Abstract

Light and bacteria can be used in combination to enhance secondary metabolite production during fermentation. Red yeast rice powder (RYRP) was inoculated with Bacillus subtilis (B2) isolated from freshwater seafood and incubated under light-emitting diodes (LEDs) of different colors (blue, green, red, white), fluorescent white light, and in darkness. Blue LED-mediated fermentation with B2 significantly enhanced production of phenolic compounds (68.4 ± 1 mg GAE/g DW) and flavonoids (51.7 ± 1 mg QE/g DW) compared to white light and darkness. Total antioxidant activity of RYRP extract after fermentation with B2 was > 77%; hydroxyl radical and superoxide scavenging were > 66%. DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) radical scavenging activities were 51% and > 67%, respectively. Reducing power was approximately twice that of extract from RYRP without B2. FTIR analysis showed a high content of hydroxyl, nitrile and carboxylic groups in the extract. Derivatives of cinnamic, benzoic and phophinodithioic acid, and quinazolinone were identified by GC–MS. Findings show that fermenting RYRP with B. subtilis B2 under blue LEDs enhances production of secondary metabolites, which should have applications in industrial fermentation processes.

Original languageEnglish (US)
Pages (from-to)529-539
Number of pages11
JournalBioprocess and Biosystems Engineering
Volume42
Issue number4
DOIs
StatePublished - Apr 9 2019

Fingerprint

Manuscripts
Bacilli
Bacillus subtilis
Antioxidants
Yeast
Powders
Fermentation
Light emitting diodes
Light
Scavenging
Metabolites
Hydroxyl Radical
Darkness
Quinazolinones
Flavonoids
Nitriles
Benzoic Acid
Sulfonic Acids
Acids
Hydrates

Keywords

  • Antioxidant
  • Bacillus subtilis
  • Light-emitting diodes
  • Phenolic compounds
  • Red yeast rice powder

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Red yeast rice fermentation with Bacillus subtilis B2 under blue light-emitting diodes increases antioxidant secondary products (Manuscript ID : BPBSE-18-0387). / Elumalai, Punniyakotti; Park, Yool Jin; Cho, Min; Shea, Patrick J; Oh, Byung Taek.

In: Bioprocess and Biosystems Engineering, Vol. 42, No. 4, 09.04.2019, p. 529-539.

Research output: Contribution to journalArticle

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abstract = "Light and bacteria can be used in combination to enhance secondary metabolite production during fermentation. Red yeast rice powder (RYRP) was inoculated with Bacillus subtilis (B2) isolated from freshwater seafood and incubated under light-emitting diodes (LEDs) of different colors (blue, green, red, white), fluorescent white light, and in darkness. Blue LED-mediated fermentation with B2 significantly enhanced production of phenolic compounds (68.4 ± 1 mg GAE/g DW) and flavonoids (51.7 ± 1 mg QE/g DW) compared to white light and darkness. Total antioxidant activity of RYRP extract after fermentation with B2 was > 77{\%}; hydroxyl radical and superoxide scavenging were > 66{\%}. DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) radical scavenging activities were 51{\%} and > 67{\%}, respectively. Reducing power was approximately twice that of extract from RYRP without B2. FTIR analysis showed a high content of hydroxyl, nitrile and carboxylic groups in the extract. Derivatives of cinnamic, benzoic and phophinodithioic acid, and quinazolinone were identified by GC–MS. Findings show that fermenting RYRP with B. subtilis B2 under blue LEDs enhances production of secondary metabolites, which should have applications in industrial fermentation processes.",
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