Red blood cells stimulate human lung fibroblasts to secrete interleukin-8

K. Fredriksson, J. Lundahl, L. Palmberg, Debra Romberger, Xiang-de Liu, S. I. Rennard, C. M. Skold

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Following lung injury, red blood cells (RBC) may interact with extracellular matrix (ECM). Fibroblasts, the resident cell in the ECM, have the capacity to produce and secrete a variety of mediators including interleukin-8 (IL-8). In the present study we hypothesized that RBC, or soluble factors released from them, may stimulate IL-8 production by fibroblasts. Fibroblasts were cultured in a three-dimensional collagen gel culture system in the presence or absence of RBC or conditioned medium from RBC (RBC-CM). IL-8 release from fibroblasts was significantly increased when cultured with RBC or RBC-CM and both tumor necrosis factor-alpha (TNF-α) and interleukin-1β (IL- 1β) further stimulated this IL-8 secretion. The enhanced production of IL-8 within fibroblasts was accompanied by increased IL-8 mRNA expression. To evaluate whether RBC-fibroblast interaction may lead to recruitment of neutrophils, a functional migration assay was performed. RBC and RBC-CM, in the presence of IL-1β and TNF-α, increased the transmigration of neutrophils. Our results indicate that RBC, when interacting with ECM, may participate in the recruitment of inflammatory cells by stimulating fibroblasts to secrete IL-8. This might be an important mechanism regulating tissue repair after injury.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalInflammation
Volume27
Issue number2
DOIs
StatePublished - Apr 1 2003

Fingerprint

Interleukin-8
Fibroblasts
Erythrocytes
Lung
Extracellular Matrix
Interleukin-1
Tumor Necrosis Factor-alpha
Neutrophil Infiltration
Lung Injury
Conditioned Culture Medium
Cell Communication
Neutrophils
Collagen
Gels
Messenger RNA
Wounds and Injuries

Keywords

  • Collagen gel
  • Fibroblast
  • IL-8
  • Migration
  • Neutrophil

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Fredriksson, K., Lundahl, J., Palmberg, L., Romberger, D., Liu, X., Rennard, S. I., & Skold, C. M. (2003). Red blood cells stimulate human lung fibroblasts to secrete interleukin-8. Inflammation, 27(2), 71-78. https://doi.org/10.1023/A:1023274532456

Red blood cells stimulate human lung fibroblasts to secrete interleukin-8. / Fredriksson, K.; Lundahl, J.; Palmberg, L.; Romberger, Debra; Liu, Xiang-de; Rennard, S. I.; Skold, C. M.

In: Inflammation, Vol. 27, No. 2, 01.04.2003, p. 71-78.

Research output: Contribution to journalArticle

Fredriksson, K, Lundahl, J, Palmberg, L, Romberger, D, Liu, X, Rennard, SI & Skold, CM 2003, 'Red blood cells stimulate human lung fibroblasts to secrete interleukin-8', Inflammation, vol. 27, no. 2, pp. 71-78. https://doi.org/10.1023/A:1023274532456
Fredriksson, K. ; Lundahl, J. ; Palmberg, L. ; Romberger, Debra ; Liu, Xiang-de ; Rennard, S. I. ; Skold, C. M. / Red blood cells stimulate human lung fibroblasts to secrete interleukin-8. In: Inflammation. 2003 ; Vol. 27, No. 2. pp. 71-78.
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