Red blood cells stimulate fibroblast-mediated contraction of three dimensional collagen gels in co-culture

K. Fredriksson, J. Lundahl, E. Fernvik, Xiang-de Liu, S. I. Rennard, C. M. Sköld

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective and design: Following injury, red blood cells (RBC) may interact with extracellular matrix (ECM). In the present study we hypothesised that RBC, and soluble factors from RBC, might mediate remodelling of ECM by affecting fibroblast-mediated contraction of three dimensional collagen gels. Materials and methods: Human lung fibroblasts (HFL-1), were cultured together with isolated RBC, conditioned medium from RBC (RBC-CM) and hemolysed RBC in type I collagen gels. Gel contraction was determined by an image analyser. Results: Both RBC, RBC-CM and hemolysed RBC stimulated gel contraction by fibroblasts (P<0.001), compared to fibroblasts alone. The RBC-CM stimulated (P<0.01) gel contraction in a time and concentration dependent manner. A similar effect was observed when supernatant from hemolysed RBC was tested. The production of fibronectin was increased (P<0.01) in the co-culture system, compared to fibroblasts cultured alone. Conclusions: The present study shows that RBC can interact with mesenchymal cells in vitro. The ability of RBC to modulate fibroblast-mediated contraction in vitro, might therefore be an important mechanism regulating repair processes after injury.

Original languageEnglish (US)
Pages (from-to)245-251
Number of pages7
JournalInflammation Research
Volume51
Issue number5
DOIs
StatePublished - Jan 1 2002

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Fibroblasts
Coculture Techniques
Cell culture
Blood
Collagen
Erythrocytes
Gels
Cells
Extracellular Matrix
Wounds and Injuries
Conditioned Culture Medium
Collagen Type I
Fibronectins
Repair

Keywords

  • Erythrocyte
  • Extracellular matrix
  • Fibronectin
  • Fibrosis
  • Remodelling

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Red blood cells stimulate fibroblast-mediated contraction of three dimensional collagen gels in co-culture. / Fredriksson, K.; Lundahl, J.; Fernvik, E.; Liu, Xiang-de; Rennard, S. I.; Sköld, C. M.

In: Inflammation Research, Vol. 51, No. 5, 01.01.2002, p. 245-251.

Research output: Contribution to journalArticle

Fredriksson, K. ; Lundahl, J. ; Fernvik, E. ; Liu, Xiang-de ; Rennard, S. I. ; Sköld, C. M. / Red blood cells stimulate fibroblast-mediated contraction of three dimensional collagen gels in co-culture. In: Inflammation Research. 2002 ; Vol. 51, No. 5. pp. 245-251.
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