Recovery from short-term depression and facilitation is ultrafast and ca dependent at auditory hair cell synapses

Soyoun Cho, Geng Lin Li, Henrique von Gersdorff

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Abstract

Short-term facilitation and depression coexist at many CNS synapses. Facilitation, however, has not been fully characterized at hair cell synapses. Using paired recordings and membrane capacitance measurements we find that paired-pulse plasticity at an adult frog auditory hair cell synapse depends on pulse duration and interpulse intervals. For short 20 ms depolarizing pulses, and interpulse intervals between 15 and 50 ms, facilitation occurred when hair cells were held at -90 mV. However, hair cells held at -60 mV displayed only paired-pulse depression. Facilitation was dependent on residual free Ca 2 + levels because it was greatly reduced by the Ca 2+ buffers EGTA and BAPTA. Furthermore, low external Ca2+ augmented facilitation, whereas depression was augmented by high external Ca2+, consistent with depletion of a small pool of fast releasing synaptic vesicles. Recovery from depression had a double-exponential time course with a fast component that may reflect the rapid replenishment of a depleted vesicle pool. We suggest that hair cells held at more depolarized in vivo-like resting membrane potentials have a tonic influx of Ca2+; they are thus in a dynamic state of continuous vesicle release, pool depletion and replenishment. Further Ca2+ influx during paired-pulse stimuli then leads to depression. However, at membrane potentials of -90 mV, ongoing release and pool depletion are minimized, so facilitation is revealed at time intervals when rapid vesicle pool replenishment occurs. Finally, we propose that vesicle pool replenishment kinetics is not rate limited by vesicle endocytosis, which is too slow to influence the rapid pool replenishment process.

Original languageEnglish (US)
Pages (from-to)5682-5692
Number of pages11
JournalJournal of Neuroscience
Volume31
Issue number15
DOIs
StatePublished - Apr 13 2011

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Auditory Hair Cells
Synapses
Membrane Potentials
Synaptic Vesicles
Egtazic Acid
Endocytosis
Anura
Buffers
Membranes

ASJC Scopus subject areas

  • Neuroscience(all)

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Recovery from short-term depression and facilitation is ultrafast and ca dependent at auditory hair cell synapses. / Cho, Soyoun; Li, Geng Lin; von Gersdorff, Henrique.

In: Journal of Neuroscience, Vol. 31, No. 15, 13.04.2011, p. 5682-5692.

Research output: Contribution to journalArticle

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