Recombination between defective tombusvirus RNAs generates functional hybrid genomes

K. Andrew White, T. Jack Morris

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The tombusviruses represent a group of small icosahedral plant viruses that contain monopartite positive-sense RNA genomes. Tombusviruses are able to generate small replicating deletion mutants of their genomes (i.e., defective interfering RNAs) during infections via RNA recombination and/or rearrangement. To further study the process of RNA recombination and to determine whether tombusviruses were capable of trans-recombination, protoplasts were coinoculated with in vitro-generated transcripts of a nonreplicating 3'-truncated genomic RNA of cucumber necrosis tombusvirus and either replicative or replication-defective DI RNAs of tomato bushy stunt tombusvirus. After a 48-hr incubation, two dominant replicative chimeric recombinant viral RNA populations were detected that contained various large contiguous 5' segments of the cucumber necrosis tombusvirus genomic RNA fused to 3'-terminal regions of the tomato bushy stunt tombusvirus defective interfering RNA. Some of the larger chimeric recombinants formed in protoplasts were able to systemically infect plants and induce wild-type symptoms. In addition, a functional chimeric genome was generated in planta after direct coinoculation of whole plants with the defective RNA components. These results indicate that (i) RNA recombination can occur relatively efficiently in single-cell infections, (ii) trans-recombination can occur with nonreplicating viral RNA components, and (iii) functional chimeric genomes can be generated via recombination. Possible mechanisms for the formation of the recombinants are proposed, and evolutionary implications are discussed.

Original languageEnglish (US)
Pages (from-to)3642-3646
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number9
DOIs
StatePublished - Apr 26 1994

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Tombusvirus
Genetic Recombination
Genome
RNA
Cucumis sativus
Protoplasts
Viral RNA
Lycopersicon esculentum
Necrosis
Plant Viruses
Viral Structures
Infection

Keywords

  • defective interfering RNAs
  • plant virus
  • protoplasts

ASJC Scopus subject areas

  • General

Cite this

Recombination between defective tombusvirus RNAs generates functional hybrid genomes. / White, K. Andrew; Morris, T. Jack.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 9, 26.04.1994, p. 3642-3646.

Research output: Contribution to journalArticle

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