Recombinant plasmid expressing the entire coding region of the Bmyc putative protein.

M. Steinitz, S. Ingvarsson, C. Asker, Janos Sumegi, G. Klein

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

An expression vector with the entire coding region of Bmyc oncogene was constructed. The longest predicted open reading frame of Bmyc (178 amino acid residues) was expressed as a fusion protein with the trpE protein (308 amino acid residues) in transformed bacteria. The newly synthesized 58 Kd protein reacted with an anti pan-myc serum. The fusion protein isolated from a preparative Western blot was used as immunogen to generate rabbit anti myc specific immune sera.

Original languageEnglish (US)
Pages (from-to)49-54
Number of pages6
JournalJournal of clinical & laboratory immunology
Volume32
Issue number2
StatePublished - Jan 1 1990

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Plasmids
Proteins
Amino Acids
Oncogenes
Open Reading Frames
Immune Sera
Western Blotting
Rabbits
Bacteria
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Steinitz, M., Ingvarsson, S., Asker, C., Sumegi, J., & Klein, G. (1990). Recombinant plasmid expressing the entire coding region of the Bmyc putative protein. Journal of clinical & laboratory immunology, 32(2), 49-54.

Recombinant plasmid expressing the entire coding region of the Bmyc putative protein. / Steinitz, M.; Ingvarsson, S.; Asker, C.; Sumegi, Janos; Klein, G.

In: Journal of clinical & laboratory immunology, Vol. 32, No. 2, 01.01.1990, p. 49-54.

Research output: Contribution to journalArticle

Steinitz, M, Ingvarsson, S, Asker, C, Sumegi, J & Klein, G 1990, 'Recombinant plasmid expressing the entire coding region of the Bmyc putative protein.', Journal of clinical & laboratory immunology, vol. 32, no. 2, pp. 49-54.
Steinitz, M. ; Ingvarsson, S. ; Asker, C. ; Sumegi, Janos ; Klein, G. / Recombinant plasmid expressing the entire coding region of the Bmyc putative protein. In: Journal of clinical & laboratory immunology. 1990 ; Vol. 32, No. 2. pp. 49-54.
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