Reclassification of Wolbachia persica as Francisella persica comb. nov. and emended description of the family Francisellaceae

Marilynn A Larson, Ufuk Nalbantoglu, Khalid Sayood, Emily B. Zentz, Regina Zing Cer, Peter Charles Iwen, Stephen C. Francesconi, Kimberly A. Bishop-Lilly, Vishwesh P. Mokashi, Anders Sjöstedt, Steven Heye Hinrichs

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Abstract

The taxonomic status of the bacterium Wolbachia persica is described, and based on the evidence presented, transfer of this species to the genus Francisella as Francisella persica comb. nov. is proposed. This reclassification is supported by data generated from genomic comparisons of W. persica ATCC VR-331T (=FSC845T=DSM 101678T) to other near neighbours, including Francisella tularensis subsp. novicida. The full-length 16S rRNA gene sequence of strain ATCC VR-331T had 98.5% nucleotide identity to the cognate gene in F. tularensis, with the highest similarity to subspecies novicida. Phylogenetic trees of full-length 16S rRNA gene, gyrA and recA sequences from species of the genera Wolbachia (class Alphaproteobacteria) and Francisella (class Gammaproteobacteria) indicated that W. persica ATCC VR-331T was most closely related to members of the genus Francisella and not Wolbachia. Local collinear blocks within the chromosome of strain ATCC VR-331T had considerable similarity with F. tularensis subsp. novicida, but not with any Wolbachia strain. The genomes of strain ATCC VR-331T and F. tularensis subsp. novicida Utah 112T (=ATCC 15482T) contained an average nucleotide identity mean of 88.72% and median of 89.18%. Importantly, the genome of strain ATCC VR-331T contained one Francisella Pathogenicity Island, similar to F. tularensis subsp. novicida, as well as the Francisella-specific gene fopA1 and F. tularensis-specific genes fopA2 and lpnA (also referred to as tul4). In contrast to the obligate intracellular genus Wolbachia, strain ATCC VR-331T and facultative intracellular Francisella can replicate in specialized cell-free media. Collectively, these results demonstrate that Wolbachia persica should be reclassified in the genus Francisella as Francisella persica comb. nov. The type strain of Francisella persica comb. nov. is ATCC VR-331T (=FSC845T=DSM 101678T). An emended description of the family Francisellaceae is also provided.

Original languageEnglish (US)
Pages (from-to)1200-1205
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number3
DOIs
StatePublished - Mar 1 2016

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aprepitant
Francisellaceae
Francisella
Wolbachia
Comb and Wattles
taxonomic revisions
Francisella tularensis
Francisella tularensis subsp. novicida
gene
genome
pathogenicity
subspecies
rRNA Genes
chromosome
genomics
genes
nucleotides
family
ribosomal RNA
Nucleotides

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Reclassification of Wolbachia persica as Francisella persica comb. nov. and emended description of the family Francisellaceae. / Larson, Marilynn A; Nalbantoglu, Ufuk; Sayood, Khalid; Zentz, Emily B.; Cer, Regina Zing; Iwen, Peter Charles; Francesconi, Stephen C.; Bishop-Lilly, Kimberly A.; Mokashi, Vishwesh P.; Sjöstedt, Anders; Hinrichs, Steven Heye.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 66, No. 3, 01.03.2016, p. 1200-1205.

Research output: Contribution to journalArticle

Larson, Marilynn A ; Nalbantoglu, Ufuk ; Sayood, Khalid ; Zentz, Emily B. ; Cer, Regina Zing ; Iwen, Peter Charles ; Francesconi, Stephen C. ; Bishop-Lilly, Kimberly A. ; Mokashi, Vishwesh P. ; Sjöstedt, Anders ; Hinrichs, Steven Heye. / Reclassification of Wolbachia persica as Francisella persica comb. nov. and emended description of the family Francisellaceae. In: International Journal of Systematic and Evolutionary Microbiology. 2016 ; Vol. 66, No. 3. pp. 1200-1205.
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AU - Cer, Regina Zing

AU - Iwen, Peter Charles

AU - Francesconi, Stephen C.

AU - Bishop-Lilly, Kimberly A.

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AU - Sjöstedt, Anders

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