Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture

Leon O. Murphy, Yasser H.A. Abdel-Wahab, Qiming J. Wang, Joseph A. Knezetic, Johan Permert, Jörgen Larsson, Michael A Hollingsworth, Thomas E. Adrian

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Overexpression of autocrine growth factors and their receptors has been reported in many human cancers. The study of autocrine-regulated pathways using in vitro culture systems can be hindered by the presence of fetal bovine serum in culture medium. A human pancreatic cancer cell line (HPAF) was slowly weaned from its dependence on fetal bovine serum and subsequently maintained in serum-free conditions. Growth factor secretion studies showed that production of autocrine growth factors such as transforming growth factor α, gastrin-releasing peptide, and insulin-like growth factor I from weaned cells increased three times compared with nonweaned cells (p < 0.01). The epidermal growth factor and gastrin-releasing peptide receptor densities were also increased in weaned cells (2 times and 2.5 times, respectively, p < 0.05). The proliferation of weaned cells cultured continuously in the same medium was significantly greater than of nonweaned cells (p < 0.05). Collectively, these data indicate that weaned pancreatic cancer cells can proliferate in the absence of serum by up-regulating autocrine pathways.

Original languageEnglish (US)
Pages (from-to)293-298
Number of pages6
JournalPancreas
Volume22
Issue number3
DOIs
StatePublished - Apr 7 2001

Fingerprint

Pancreatic Neoplasms
Ligands
Growth
Serum
Intercellular Signaling Peptides and Proteins
Bombesin Receptors
Gastrin-Releasing Peptide
Growth Factor Receptors
Transforming Growth Factors
Insulin-Like Growth Factor I
Epidermal Growth Factor
Culture Media
Cultured Cells
Cell Proliferation
Cell Line
Neoplasms

Keywords

  • Autocrine growth factor
  • EGF
  • Pancreatic cancer
  • TGFα

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

Cite this

Murphy, L. O., Abdel-Wahab, Y. H. A., Wang, Q. J., Knezetic, J. A., Permert, J., Larsson, J., ... Adrian, T. E. (2001). Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture. Pancreas, 22(3), 293-298. https://doi.org/10.1097/00006676-200104000-00011

Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture. / Murphy, Leon O.; Abdel-Wahab, Yasser H.A.; Wang, Qiming J.; Knezetic, Joseph A.; Permert, Johan; Larsson, Jörgen; Hollingsworth, Michael A; Adrian, Thomas E.

In: Pancreas, Vol. 22, No. 3, 07.04.2001, p. 293-298.

Research output: Contribution to journalArticle

Murphy, Leon O. ; Abdel-Wahab, Yasser H.A. ; Wang, Qiming J. ; Knezetic, Joseph A. ; Permert, Johan ; Larsson, Jörgen ; Hollingsworth, Michael A ; Adrian, Thomas E. / Receptors and ligands for autocrine growth pathways are up-regulated when pancreatic cancer cells are adapted to serum-free culture. In: Pancreas. 2001 ; Vol. 22, No. 3. pp. 293-298.
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