Recent advances in chromatographic and electrophoretic methods for the study of drug-protein interactions

David S. Hage, Stacey A. Tweed

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Drug-protein binding is an important process in determining the activity and fate of a pharmaceutical agent once it has entered the body. This review examines various chromatographic and electrophoretic methods that have been developed to study such interactions. An overview of each technique is presented along with a discussion of its strengths, weaknesses and potential applications. Formats that are discussed include the use of both soluble and immobilized drugs or proteins, and approaches based on zonal elution, frontal analysis or vacancy peak measurements. Furthermore, examples are provided that illustrate the use of these methods in determining the overall extent of drug-protein binding, in examining the displacement of a drug by other agents and in measuring the equilibrium or rate constants for drug-protein interactions. Examples are also given demonstrating how the same methods, particularly when used in high-performance liquid chromatography or capillary electrophoresis systems, can be employed as rapid screening tools for investigating the binding of different forms of a chiral drug to a protein or the binding of different proteins and peptides to a given pharmaceutical agent.

Original languageEnglish (US)
Pages (from-to)499-525
Number of pages27
JournalJournal of Chromatography B: Biomedical Applications
Volume699
Issue number1-2
DOIs
StatePublished - Oct 10 1997

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Pharmaceutical Preparations
Proteins
Capillary electrophoresis
Equilibrium constants
High performance liquid chromatography
Vacancies
Rate constants
Carrier Proteins
Screening
Peptides
Protein Binding

Keywords

  • Drug-protein interactions
  • Drugs
  • Proteins
  • Reviews

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Recent advances in chromatographic and electrophoretic methods for the study of drug-protein interactions. / Hage, David S.; Tweed, Stacey A.

In: Journal of Chromatography B: Biomedical Applications, Vol. 699, No. 1-2, 10.10.1997, p. 499-525.

Research output: Contribution to journalArticle

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