Rearrangement and expression of T-cell receptor genes in marmoset lymphoid cell lines with natural killer activity

M. Stevenson, Donald R Johnson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To study the role of the T-3/T-cell receptor complex in mediating natural killer activity, we have examined T-cell receptor gene configuration and expression in five transformed marmoset cell lines with natural killer activity. All lines showed rearrangement of the beta chain of the T-cell receptor and expressed full size beta chain transcripts as well as full size delta chain transcripts. Only three lines contained full size T-cell receptor alpha chain transcripts. Of the three lines which had potential for encoding functional T-3/T-cell receptor complex, those which possessed natural killer activity also expressed T-gamma transcripts. In addition, one clone with high natural killer activity which did express high levels of T-gamma mRNA was unable to produce functional T-3/T-cell receptor complex due to truncated T-cell receptor alpha chain message. The results in this paper provide strong evidence that natural killer activity is dependent upon expression of the T-cell receptor gamma chain.

Original languageEnglish (US)
Pages (from-to)9-17
Number of pages9
JournalViral Immunology
Volume3
Issue number1
DOIs
StatePublished - Jan 1 1990

Fingerprint

Antigen Receptors, T-Cell, alpha-beta
T-Cell Receptor Genes
Callithrix
T-Cell Antigen Receptor
Lymphocytes
Cell Line
Antigen Receptors, T-Cell, gamma-delta
Transformed Cell Line
Clone Cells
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

Cite this

Rearrangement and expression of T-cell receptor genes in marmoset lymphoid cell lines with natural killer activity. / Stevenson, M.; Johnson, Donald R.

In: Viral Immunology, Vol. 3, No. 1, 01.01.1990, p. 9-17.

Research output: Contribution to journalArticle

@article{473a6c0a481f488c8f8277e217efcf05,
title = "Rearrangement and expression of T-cell receptor genes in marmoset lymphoid cell lines with natural killer activity",
abstract = "To study the role of the T-3/T-cell receptor complex in mediating natural killer activity, we have examined T-cell receptor gene configuration and expression in five transformed marmoset cell lines with natural killer activity. All lines showed rearrangement of the beta chain of the T-cell receptor and expressed full size beta chain transcripts as well as full size delta chain transcripts. Only three lines contained full size T-cell receptor alpha chain transcripts. Of the three lines which had potential for encoding functional T-3/T-cell receptor complex, those which possessed natural killer activity also expressed T-gamma transcripts. In addition, one clone with high natural killer activity which did express high levels of T-gamma mRNA was unable to produce functional T-3/T-cell receptor complex due to truncated T-cell receptor alpha chain message. The results in this paper provide strong evidence that natural killer activity is dependent upon expression of the T-cell receptor gamma chain.",
author = "M. Stevenson and Johnson, {Donald R}",
year = "1990",
month = "1",
day = "1",
doi = "10.1089/vim.1990.3.9",
language = "English (US)",
volume = "3",
pages = "9--17",
journal = "Viral Immunology",
issn = "0882-8245",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - Rearrangement and expression of T-cell receptor genes in marmoset lymphoid cell lines with natural killer activity

AU - Stevenson, M.

AU - Johnson, Donald R

PY - 1990/1/1

Y1 - 1990/1/1

N2 - To study the role of the T-3/T-cell receptor complex in mediating natural killer activity, we have examined T-cell receptor gene configuration and expression in five transformed marmoset cell lines with natural killer activity. All lines showed rearrangement of the beta chain of the T-cell receptor and expressed full size beta chain transcripts as well as full size delta chain transcripts. Only three lines contained full size T-cell receptor alpha chain transcripts. Of the three lines which had potential for encoding functional T-3/T-cell receptor complex, those which possessed natural killer activity also expressed T-gamma transcripts. In addition, one clone with high natural killer activity which did express high levels of T-gamma mRNA was unable to produce functional T-3/T-cell receptor complex due to truncated T-cell receptor alpha chain message. The results in this paper provide strong evidence that natural killer activity is dependent upon expression of the T-cell receptor gamma chain.

AB - To study the role of the T-3/T-cell receptor complex in mediating natural killer activity, we have examined T-cell receptor gene configuration and expression in five transformed marmoset cell lines with natural killer activity. All lines showed rearrangement of the beta chain of the T-cell receptor and expressed full size beta chain transcripts as well as full size delta chain transcripts. Only three lines contained full size T-cell receptor alpha chain transcripts. Of the three lines which had potential for encoding functional T-3/T-cell receptor complex, those which possessed natural killer activity also expressed T-gamma transcripts. In addition, one clone with high natural killer activity which did express high levels of T-gamma mRNA was unable to produce functional T-3/T-cell receptor complex due to truncated T-cell receptor alpha chain message. The results in this paper provide strong evidence that natural killer activity is dependent upon expression of the T-cell receptor gamma chain.

UR - http://www.scopus.com/inward/record.url?scp=0025104095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025104095&partnerID=8YFLogxK

U2 - 10.1089/vim.1990.3.9

DO - 10.1089/vim.1990.3.9

M3 - Article

VL - 3

SP - 9

EP - 17

JO - Viral Immunology

JF - Viral Immunology

SN - 0882-8245

IS - 1

ER -