Rational design of a secreted enzymatically inactive mutant of extracellular superoxide dismutase

Adam J. Case, James J. Mezhir, Brianne R. O'Leary, Jennifer E. Hrabe, Frederick E. Domann

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Extracellular superoxide dismutase (SOD3) is a secreted enzyme that regulates levels of extracellular superoxide and protects the extracellular matrix from degradation by reactive species. The SOD3 protein contains a heparin-binding domain and resides in a microenvironment rich in other heparin-bound growth factors, raising the possibility that SOD3 may have some biological role independent of its catalytic activity. To begin to address this, we designed and created enzymatically inactive mutant constructs targeting either the copper coordinating (i.e. H96 and H98) or superoxide channeling (i.e. N180 and R186) amino acid residues of SOD3. All constructs expressed equal quantities of immature intracellular SOD proteins, but only the N180A, R186A, and combination N180A/R186A mutants produced fully processed and secreted extracellular protein. Furthermore, while SOD activity was significantly inhibited in the single N180A and R186A mutants, the activity was completely abrogated in the N180A/R186A double mutant. Overall, the use of this novel tool may have broad reaching impacts into various fields of biology and medicine, and will aid in the delineation of cellular processes that are regulated by solely the SOD3 protein, its reactive oxygen species substrates and products, or the combination of both.

Original languageEnglish (US)
Pages (from-to)239-245
Number of pages7
JournalRedox Report
Volume17
Issue number6
DOIs
StatePublished - Dec 1 2012

Fingerprint

Superoxide Dismutase
Superoxides
Heparin
Proteins
Medicine
Extracellular Matrix
Copper
Reactive Oxygen Species
Catalyst activity
Intercellular Signaling Peptides and Proteins
Amino Acids
Degradation
Substrates
Enzymes

Keywords

  • Antioxidants
  • Free radicals
  • Heparan sulfate proteoglycans
  • Oxidative stress
  • Signaling proteins

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical

Cite this

Rational design of a secreted enzymatically inactive mutant of extracellular superoxide dismutase. / Case, Adam J.; Mezhir, James J.; O'Leary, Brianne R.; Hrabe, Jennifer E.; Domann, Frederick E.

In: Redox Report, Vol. 17, No. 6, 01.12.2012, p. 239-245.

Research output: Contribution to journalArticle

Case, Adam J. ; Mezhir, James J. ; O'Leary, Brianne R. ; Hrabe, Jennifer E. ; Domann, Frederick E. / Rational design of a secreted enzymatically inactive mutant of extracellular superoxide dismutase. In: Redox Report. 2012 ; Vol. 17, No. 6. pp. 239-245.
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