Ratiometric Fluorescent Sensor Array as a Versatile Tool for Bacterial Pathogen Identification and Analysis

Denis Svechkarev, Marat R. Sadykov, Kenneth W. Bayles, Aaron M. Mohs

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Rapid and reliable identification of pathogenic microorganisms is of great importance for human and animal health. Most conventional approaches are time-consuming and require expensive reagents, sophisticated equipment, trained personnel, and special storage and handling conditions. Sensor arrays based on small molecules offer a chemically stable and cost-effective alternative. Here we present a ratiometric fluorescent sensor array based on the derivatives of 2-(4′-N,N-dimethylamino)-3-hydroxyflavone and investigate its ability to provide a dual-channel ratiometric response. We demonstrate that, by using discriminant analysis of the sensor array responses, it is possible to effectively distinguish between eight bacterial species and recognize their Gram status. Thus, multiple parameters can be derived from the same data set. Moreover, the predictive potential of this sensor array is discussed, and its ability to analyze unknown samples beyond the list of species used for the training matrix is demonstrated. The proposed sensor array and analysis strategies open new avenues for the development of advanced ratiometric sensors for multiparametric analysis.

Original languageEnglish (US)
Pages (from-to)700-708
Number of pages9
JournalACS Sensors
Volume3
Issue number3
DOIs
StatePublished - Mar 23 2018

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Keywords

  • 3-hydroxyflavone
  • ESIPT
  • Gram status
  • chemical nose
  • discriminant analysis
  • multiparametric sensing
  • pattern analysis
  • predictive analysis

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes

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