Rate of growth and extent of differentiation reflected by cytoplasmic proteins and antigens of human colon tumor cell lines

C. W. Taylor, M. G. Brattain, L. C. Yeoman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The cytosolic proteins and antigens of 11 human colon tumor cell lines were examined with respect to their rate of growth and state of differentiation. Coomassie blue-stained protein analysis of sodium dodecyl sulfate-containing polyacrylamide gels revealed protein bands at M(r) 30,000, 31,000, and 58,000, which were characteristic of slower growing and more differentiated cell lines. More rapidly dividing and disdifferentiated colon cell lines lacked the M(r) 30,000 and M(r) 58,000 bands; instead, they produced a single protein band that ran between the M(r) 30,000 and M(r) 31,000 positions on the gel. Western transfer analysis of cytoplasmic antigens further subdivided the 11 cell lines into 3 separate categories. Slowly growing and more differentiated lines produced a M(r) 52,000 antigen. Intermediate lines, with respect to growth rate and state of differentiation, produced a M(r) 38,000 antigen. The rapidly growing and highly disdifferentiated cell lines contained three cytosolic antigens with molecular weights of 37,000, 39,000, and 48,000. These criteria made it possible to classify these 11 human colon tumor tissue culture cell lines into 3 groups which reflect their state of growth activity and degree of differentiation.

Original languageEnglish (US)
Pages (from-to)1200-1205
Number of pages6
JournalCancer Research
Volume44
Issue number3
StatePublished - 1984
Externally publishedYes

Fingerprint

Tumor Cell Line
Colon
Antigens
Cell Line
Growth
Proteins
ran GTP-Binding Protein
Connectin
Sodium Dodecyl Sulfate
Molecular Weight
Gels
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Rate of growth and extent of differentiation reflected by cytoplasmic proteins and antigens of human colon tumor cell lines. / Taylor, C. W.; Brattain, M. G.; Yeoman, L. C.

In: Cancer Research, Vol. 44, No. 3, 1984, p. 1200-1205.

Research output: Contribution to journalArticle

Taylor, C. W. ; Brattain, M. G. ; Yeoman, L. C. / Rate of growth and extent of differentiation reflected by cytoplasmic proteins and antigens of human colon tumor cell lines. In: Cancer Research. 1984 ; Vol. 44, No. 3. pp. 1200-1205.
@article{4fb5a93f6085469493e3c35abb1e49e1,
title = "Rate of growth and extent of differentiation reflected by cytoplasmic proteins and antigens of human colon tumor cell lines",
abstract = "The cytosolic proteins and antigens of 11 human colon tumor cell lines were examined with respect to their rate of growth and state of differentiation. Coomassie blue-stained protein analysis of sodium dodecyl sulfate-containing polyacrylamide gels revealed protein bands at M(r) 30,000, 31,000, and 58,000, which were characteristic of slower growing and more differentiated cell lines. More rapidly dividing and disdifferentiated colon cell lines lacked the M(r) 30,000 and M(r) 58,000 bands; instead, they produced a single protein band that ran between the M(r) 30,000 and M(r) 31,000 positions on the gel. Western transfer analysis of cytoplasmic antigens further subdivided the 11 cell lines into 3 separate categories. Slowly growing and more differentiated lines produced a M(r) 52,000 antigen. Intermediate lines, with respect to growth rate and state of differentiation, produced a M(r) 38,000 antigen. The rapidly growing and highly disdifferentiated cell lines contained three cytosolic antigens with molecular weights of 37,000, 39,000, and 48,000. These criteria made it possible to classify these 11 human colon tumor tissue culture cell lines into 3 groups which reflect their state of growth activity and degree of differentiation.",
author = "Taylor, {C. W.} and Brattain, {M. G.} and Yeoman, {L. C.}",
year = "1984",
language = "English (US)",
volume = "44",
pages = "1200--1205",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "3",

}

TY - JOUR

T1 - Rate of growth and extent of differentiation reflected by cytoplasmic proteins and antigens of human colon tumor cell lines

AU - Taylor, C. W.

AU - Brattain, M. G.

AU - Yeoman, L. C.

PY - 1984

Y1 - 1984

N2 - The cytosolic proteins and antigens of 11 human colon tumor cell lines were examined with respect to their rate of growth and state of differentiation. Coomassie blue-stained protein analysis of sodium dodecyl sulfate-containing polyacrylamide gels revealed protein bands at M(r) 30,000, 31,000, and 58,000, which were characteristic of slower growing and more differentiated cell lines. More rapidly dividing and disdifferentiated colon cell lines lacked the M(r) 30,000 and M(r) 58,000 bands; instead, they produced a single protein band that ran between the M(r) 30,000 and M(r) 31,000 positions on the gel. Western transfer analysis of cytoplasmic antigens further subdivided the 11 cell lines into 3 separate categories. Slowly growing and more differentiated lines produced a M(r) 52,000 antigen. Intermediate lines, with respect to growth rate and state of differentiation, produced a M(r) 38,000 antigen. The rapidly growing and highly disdifferentiated cell lines contained three cytosolic antigens with molecular weights of 37,000, 39,000, and 48,000. These criteria made it possible to classify these 11 human colon tumor tissue culture cell lines into 3 groups which reflect their state of growth activity and degree of differentiation.

AB - The cytosolic proteins and antigens of 11 human colon tumor cell lines were examined with respect to their rate of growth and state of differentiation. Coomassie blue-stained protein analysis of sodium dodecyl sulfate-containing polyacrylamide gels revealed protein bands at M(r) 30,000, 31,000, and 58,000, which were characteristic of slower growing and more differentiated cell lines. More rapidly dividing and disdifferentiated colon cell lines lacked the M(r) 30,000 and M(r) 58,000 bands; instead, they produced a single protein band that ran between the M(r) 30,000 and M(r) 31,000 positions on the gel. Western transfer analysis of cytoplasmic antigens further subdivided the 11 cell lines into 3 separate categories. Slowly growing and more differentiated lines produced a M(r) 52,000 antigen. Intermediate lines, with respect to growth rate and state of differentiation, produced a M(r) 38,000 antigen. The rapidly growing and highly disdifferentiated cell lines contained three cytosolic antigens with molecular weights of 37,000, 39,000, and 48,000. These criteria made it possible to classify these 11 human colon tumor tissue culture cell lines into 3 groups which reflect their state of growth activity and degree of differentiation.

UR - http://www.scopus.com/inward/record.url?scp=0021352192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021352192&partnerID=8YFLogxK

M3 - Article

VL - 44

SP - 1200

EP - 1205

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 3

ER -