Rat urinary bladder epithelial lesions induced by acrolein

T. Sakata, R. A. Smith, E. M. Garland, Samuel Monroe Cohen

Research output: Contribution to journalArticle

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Abstract

Acrolein, a constituent of cigarette smoke and a metabolite of cyclophosphamide, has been shown to induce acute cytotoxicity of the rat urinary bladder mucosa when instilled directly into the bladder lumen. To evaluate the effects of systemic administration, we examined the rat urinary bladder following intragastric or intraperitoneal administration of acrolein to male F344 rats. In an initial experiment, acrolein was administered at a dose of 25 mg/kg, which proved to be extremely toxic. Five of 12 rats injected intragastrically and 5 of 12 injected intraperitoneally died within 24 hrs. After 2 days, 3 of the 3 surviving rats injected intraperitoneally had focal simple hyperplasia of the urinary bladder. None of the rats injected intragastrically had bladder hyperplasia. In a second experiment, acrolein was administered by intraperitoneal injection at doses of 0.5, 1, 2, 4, and 6 mg/kg. Five days later, the labeling index of the bladder mucosa was evaluated by autoradiography. In rats injected with 6 mg/kg of acrolein, the labeling index was significantly increased compared to the other doses and compared to a vehicle injection control group. These data indicate that sufficient acrolein reaches the urinary bladder to induce a proliferative response following intraperitoneal administration.

Original languageEnglish (US)
Pages (from-to)159-169
Number of pages11
JournalJournal of Environmental Pathology, Toxicology and Oncology
Volume9
Issue number2
StatePublished - Jan 1 1989

Fingerprint

Acrolein
lesion
Rats
Urinary Bladder
smoke
metabolite
Labeling
Hyperplasia
experiment
Mucous Membrane
Poisons
Inbred F344 Rats
Cytotoxicity
Metabolites
dose
Intraperitoneal Injections
Autoradiography
Smoke
Tobacco Products
Cyclophosphamide

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Rat urinary bladder epithelial lesions induced by acrolein. / Sakata, T.; Smith, R. A.; Garland, E. M.; Cohen, Samuel Monroe.

In: Journal of Environmental Pathology, Toxicology and Oncology, Vol. 9, No. 2, 01.01.1989, p. 159-169.

Research output: Contribution to journalArticle

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