Rat liver cytosolic 4 S polycyclic aromatic hydrocarbon-binding protein is glycine N-methyltransferase

Abhijit Raha, Conrad Wagner, Richard G MacDonald, Edward Bresnick

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In the rat, cytochrome P-450IA1 gene expression, which is most closely associated with aryl hydrocarbon hydroxylase activity, is thought to be regulated by several trans-acting factors, including the 4 S polycyclic aromatic hydrocarbon (PAH)-binding protein. This protein has been purified to homogeneity from rat liver using ion exchange, gel permeation, hydrophobic interaction, and affinity chromatographies. Partial sequencing of the 33-kDa band indicated its identity as glycine N-methyltransferase (GNMT). Polyclonal antibodies to GNMT immunoprecipitated PAH-binding activity from rat liver cytosol. Methyltransferase and PAH-binding activities copurified during the course of protein purification. GNMT protein and PAH binding activity were colocalized in various cytosolic fractions. These data all indicate that the 4 S PAH-binding protein and GNMT are one and the same protein or very similar proteins. Western blot analyses yielded a positive signal under denaturing (33 kDa) and nondenaturing (150 kDa, tetramer) conditions; the PAH-binding protein also was an oligomer. GNMT was detected by immunohistochemistry in nuclei from H4IIE rat hepatoma cells and rat liver. The localization of GNMT in liver nuclei is in accordance with a role in modulating cytochrome P- 450IA1 gene expression.

Original languageEnglish (US)
Pages (from-to)5750-5756
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number8
StatePublished - Feb 25 1994

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Glycine N-Methyltransferase
Polycyclic Aromatic Hydrocarbons
Liver
Rats
Carrier Proteins
Cytochromes
Proteins
Gene expression
Aryl Hydrocarbon Hydroxylases
Affinity chromatography
Gene Expression
Trans-Activators
Ion Exchange
Methyltransferases
Hydrophobic and Hydrophilic Interactions
Affinity Chromatography
Oligomers
Permeation
Cytosol
Purification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Rat liver cytosolic 4 S polycyclic aromatic hydrocarbon-binding protein is glycine N-methyltransferase. / Raha, Abhijit; Wagner, Conrad; MacDonald, Richard G; Bresnick, Edward.

In: Journal of Biological Chemistry, Vol. 269, No. 8, 25.02.1994, p. 5750-5756.

Research output: Contribution to journalArticle

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