Rat gastric gelatinase induction during endotoxemia

Emily K. Robinson, Sonlee D. West, Alian Garay, David W. Mercer

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Despite continued investigation, the pathogenesis of tissue injury secondary to sepsis remains elusive. Further evaluation of the mechanisms by which endotoxemia and sepsis induce tissue injury is necessary to formulate rational and effective treatment strategies. The purpose of these studies was to evaluate the role of the matrix metalloproteinases MMP-2 and MMP-9 in gastric injury during lipopolysaccharide induced endotoxemia. Lipopolysaccharide increased gastric gelatinase activity as determined by in situ and gelatin zymography. Specifically, lipopolysaccharide induced MMP-2, MMP-9, and tissue inhibitor of metalloproteinase-1 (TIMP-1) transcription, with subsequent increases in MMP-2 and TIMP-1 protein expression. Furthermore, selective metalloproteinase inhibition ameliorated gastric injury in this model. These data suggest that lipopolysaccharide-induced gastric injury is mediated, at least in part, by increased MMP-2 production.

Original languageEnglish (US)
Pages (from-to)548-559
Number of pages12
JournalDigestive Diseases and Sciences
Volume51
Issue number3
DOIs
StatePublished - Mar 1 2006

Fingerprint

Gelatinases
Endotoxemia
Matrix Metalloproteinases
Stomach
Lipopolysaccharides
Wounds and Injuries
Tissue Inhibitor of Metalloproteinase-1
Sepsis
Matrix Metalloproteinase 2
Metalloproteases
Gelatin
Proteins

Keywords

  • Gelatinase
  • Lipopolysaccharide
  • Matrix metalloproteinase
  • Tissue inhibitor of metalloproteinase (TIMP)
  • Zymography

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Rat gastric gelatinase induction during endotoxemia. / Robinson, Emily K.; West, Sonlee D.; Garay, Alian; Mercer, David W.

In: Digestive Diseases and Sciences, Vol. 51, No. 3, 01.03.2006, p. 548-559.

Research output: Contribution to journalArticle

Robinson, Emily K. ; West, Sonlee D. ; Garay, Alian ; Mercer, David W. / Rat gastric gelatinase induction during endotoxemia. In: Digestive Diseases and Sciences. 2006 ; Vol. 51, No. 3. pp. 548-559.
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