Rat embryo development in vitro

Shyamal K Roy, J. Sengupta, S. K. Manchanda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We report the first successful culture of 8-cell/morula stage rat embryos in a fully synthetic medium supplemented with 3% crystalline bovine serum albumin. Eighty-four per cent of morulae developed to blastocysts, showing that this is a highly efficient culture medium for in vitro studies on rat pre-implantation embryos. Blastocyst formation was severely inhibited by antioestrogen (nafoxidine 3 μg/ml) but no further reversal was obtained by giving oestrogen to culture medium containing this antagonist.

Original languageEnglish (US)
Pages (from-to)546-551
Number of pages6
JournalActa Endocrinologica
Volume96
Issue number4
StatePublished - Jan 1 1981

Fingerprint

Morula
Blastocyst
Embryonic Development
Culture Media
Nafoxidine
Estrogen Receptor Modulators
Bovine Serum Albumin
Estrogens
Embryonic Structures
Cell Culture Techniques
In Vitro Techniques

ASJC Scopus subject areas

  • Endocrinology

Cite this

Roy, S. K., Sengupta, J., & Manchanda, S. K. (1981). Rat embryo development in vitro. Acta Endocrinologica, 96(4), 546-551.

Rat embryo development in vitro. / Roy, Shyamal K; Sengupta, J.; Manchanda, S. K.

In: Acta Endocrinologica, Vol. 96, No. 4, 01.01.1981, p. 546-551.

Research output: Contribution to journalArticle

Roy, SK, Sengupta, J & Manchanda, SK 1981, 'Rat embryo development in vitro', Acta Endocrinologica, vol. 96, no. 4, pp. 546-551.
Roy SK, Sengupta J, Manchanda SK. Rat embryo development in vitro. Acta Endocrinologica. 1981 Jan 1;96(4):546-551.
Roy, Shyamal K ; Sengupta, J. ; Manchanda, S. K. / Rat embryo development in vitro. In: Acta Endocrinologica. 1981 ; Vol. 96, No. 4. pp. 546-551.
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