Rapid transition of cardiac myocytes from hyperplasia to hypertrophy during postnatal development

Faqian Li, Xuejun Wang, Joseph M. Capasso, A. Martin Gerdes

Research output: Contribution to journalArticle

513 Citations (Scopus)

Abstract

The switch from myocyte hyperplasia to hypertrophy occurs during the early postnatal period. The exact temporal sequence when cardiac myocytes cease dividing and become terminally differentiated is not certain, although it is currently believed that the transition takes place gradually over a 1-2-week period. The present investigation has characterized the growth pattern of cardiac myocytes during the early postnatal period. Cardiac myocytes were enzymatically isolated from the hearts of 1, 2, 3, 4, 6, 8, 10, and 12-day-old rats for the measurements of binucleation, cell volume and myocyte number. Almost all myocytes were mononucleated and cell volume remained relatively constant during the first 3 days of age. Increases in cell volume and binucleation of myocytes were first detected at day 4. Myocyte volume increased 2.5-fold from day 3 to day 12 (1416 ± 320 compared to 3533 ± 339 μm3). The percentage of binucleated myocytes began to increase at day 4 and proceeded at a high rate, reaching the adult level of approximately 90% at day 12. Myocyte number increased 68% during the first 3 days (from 13.6 ± 3.5 x 106 at day 1 to 2219 ± 5.6 x 1010 at day 3) and remained constant thereafter. To confirm that no further myocyte division exists after 4 days, bromodeoxyuridine (Brdu) was administered to 4-day-old rats and the fate of DNA-synthesizing myocytes was examined 2 h and 2, 4, 6 and 8 days after Brdu injection. About 12% of myocytes were labeled with Brdu at 2h and all were mononucleated at that time. Gradually, these Brdu-labeled myocytes became binucleated. However, the percentage of labeled myocytes in all groups was identical, indicating that DNA-synthesizing myocytes were becoming binucleated without further cell division after 4 days of age. Within 8 days after injection, approximately 82% of total labeled myocytes were binucleated, while the others remained mononucleated. Sarcomeric α-actinin was fully disassembled in dividing myocytes of 2-day-old rats, while typical α-actinin striations were present in dividing myocytes of 4-day-old rats. The results from this study suggest that a rapid switch from myocyte hyperplasia to hypertrophy occurs between postnatal day 3 and 4 in rat hearts.

Original languageEnglish (US)
Pages (from-to)1737-1746
Number of pages10
JournalJournal of Molecular and Cellular Cardiology
Volume28
Issue number8
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

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Cardiac Myocytes
Muscle Cells
Hypertrophy
Hyperplasia
Bromodeoxyuridine
Cell Size
Actinin
Injections
DNA
Cell Division

Keywords

  • Hyperplasia
  • Hypertrophy
  • Isolated myocytes
  • Postnatal growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Rapid transition of cardiac myocytes from hyperplasia to hypertrophy during postnatal development. / Li, Faqian; Wang, Xuejun; Capasso, Joseph M.; Gerdes, A. Martin.

In: Journal of Molecular and Cellular Cardiology, Vol. 28, No. 8, 01.01.1996, p. 1737-1746.

Research output: Contribution to journalArticle

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abstract = "The switch from myocyte hyperplasia to hypertrophy occurs during the early postnatal period. The exact temporal sequence when cardiac myocytes cease dividing and become terminally differentiated is not certain, although it is currently believed that the transition takes place gradually over a 1-2-week period. The present investigation has characterized the growth pattern of cardiac myocytes during the early postnatal period. Cardiac myocytes were enzymatically isolated from the hearts of 1, 2, 3, 4, 6, 8, 10, and 12-day-old rats for the measurements of binucleation, cell volume and myocyte number. Almost all myocytes were mononucleated and cell volume remained relatively constant during the first 3 days of age. Increases in cell volume and binucleation of myocytes were first detected at day 4. Myocyte volume increased 2.5-fold from day 3 to day 12 (1416 ± 320 compared to 3533 ± 339 μm3). The percentage of binucleated myocytes began to increase at day 4 and proceeded at a high rate, reaching the adult level of approximately 90{\%} at day 12. Myocyte number increased 68{\%} during the first 3 days (from 13.6 ± 3.5 x 106 at day 1 to 2219 ± 5.6 x 1010 at day 3) and remained constant thereafter. To confirm that no further myocyte division exists after 4 days, bromodeoxyuridine (Brdu) was administered to 4-day-old rats and the fate of DNA-synthesizing myocytes was examined 2 h and 2, 4, 6 and 8 days after Brdu injection. About 12{\%} of myocytes were labeled with Brdu at 2h and all were mononucleated at that time. Gradually, these Brdu-labeled myocytes became binucleated. However, the percentage of labeled myocytes in all groups was identical, indicating that DNA-synthesizing myocytes were becoming binucleated without further cell division after 4 days of age. Within 8 days after injection, approximately 82{\%} of total labeled myocytes were binucleated, while the others remained mononucleated. Sarcomeric α-actinin was fully disassembled in dividing myocytes of 2-day-old rats, while typical α-actinin striations were present in dividing myocytes of 4-day-old rats. The results from this study suggest that a rapid switch from myocyte hyperplasia to hypertrophy occurs between postnatal day 3 and 4 in rat hearts.",
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