Rapid, sequence-specific detection of unpurified PCR amplicons via a reusable, electrochemical sensor

Rebecca Y. Lai, Eric T. Lagally, Sang Ho Lee, H. T. Soh, Kevin W. Plaxco, Alan J. Heeger

Research output: Contribution to journalArticle

157 Scopus citations

Abstract

We report an electrochemical method for the sequence-specific detection of unpurified amplification products of the gyrB gene of Salmonella typhimurium. Using an asymmetric PCR and the electrochemical E-DNA detection scheme, single-stranded amplicons were produced from as few as 90 gene copies and, without subsequent purification, rapidly identified. The detection is specific; the sensor does not respond when challenged with control oligonucleotides based on the gyrB genes of either Escherichia coli or various Shigella species. In contrast to existing sequence-specific optical- and capillary electrophoresis-based detection methods, the E-DNA sensor is fully electronic and requires neither cumbersome, expensive optics nor high voltage power supplies. Given these advantages, E-DNA sensors appear well suited for implementation in portable PCR microdevices directed at, for example, the rapid detection of pathogens.

Original languageEnglish (US)
Pages (from-to)4017-4021
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Issue number11
DOIs
Publication statusPublished - Mar 14 2006

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Keywords

  • E-DNA
  • Methylene blue
  • Polymerase chain reaction
  • Salmonella gyrB

ASJC Scopus subject areas

  • General

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