Rapid screening of testosterone in the aquatic environment using direct analysis in real-time (DART) mass spectrometry

Y. T. Lei, Y. Lu, Tian C Zhang, Y. Qi, Yongfeng Lu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Testosterone (TES) in the aquatic environment was analyzed for the first time using direct analysis in real-time mass spectrometry (DART MS) without any sample pre-treatment. TES with the addition of 2H and its dehydration products was observed. The instrument parameters were studied systematically and optimized concerning the specific analyte. Limits of detection for the pure compound were 2.5 ng for TES spiked in raw wastewater samples and 500 pg in TES standards. The whole analysis time was within 1 min. A simple derivatization of TES by hydroxylamine and introducing ammonia as dopant gas for the sensitivity and intensity enhancement were also investigated. Both methods improved the intensity of the TES signal by about twofold. The results of this study illustrate that DART MS is capable to detect TES in complicated matrixes and could realize the rapid and direct screening of TES in the aquatic environment at a low nanogram range for pure compound without coupling with any pre-treatment tools.

Original languageEnglish (US)
Article number1005
JournalEnvironmental Earth Sciences
Volume75
Issue number12
DOIs
StatePublished - Jun 1 2016

Fingerprint

testosterone
aquatic environment
Dehydration
Mass spectrometry
Testosterone
Ammonia
Screening
Wastewater
mass spectrometry
Doping (additives)
screening
Gases
pretreatment
Hydroxylamine
analysis
derivatization
dehydration
wastewater
detection limit
ammonia

Keywords

  • Ambient ionization
  • Environmental analysis
  • Steroids hormone
  • Wastewater

ASJC Scopus subject areas

  • Soil Science
  • Environmental Chemistry
  • Water Science and Technology
  • Pollution
  • Global and Planetary Change
  • Geology
  • Earth-Surface Processes

Cite this

Rapid screening of testosterone in the aquatic environment using direct analysis in real-time (DART) mass spectrometry. / Lei, Y. T.; Lu, Y.; Zhang, Tian C; Qi, Y.; Lu, Yongfeng.

In: Environmental Earth Sciences, Vol. 75, No. 12, 1005, 01.06.2016.

Research output: Contribution to journalArticle

@article{2cc2bb32d9f84a47aa0cfacc410bf9f9,
title = "Rapid screening of testosterone in the aquatic environment using direct analysis in real-time (DART) mass spectrometry",
abstract = "Testosterone (TES) in the aquatic environment was analyzed for the first time using direct analysis in real-time mass spectrometry (DART MS) without any sample pre-treatment. TES with the addition of 2H and its dehydration products was observed. The instrument parameters were studied systematically and optimized concerning the specific analyte. Limits of detection for the pure compound were 2.5 ng for TES spiked in raw wastewater samples and 500 pg in TES standards. The whole analysis time was within 1 min. A simple derivatization of TES by hydroxylamine and introducing ammonia as dopant gas for the sensitivity and intensity enhancement were also investigated. Both methods improved the intensity of the TES signal by about twofold. The results of this study illustrate that DART MS is capable to detect TES in complicated matrixes and could realize the rapid and direct screening of TES in the aquatic environment at a low nanogram range for pure compound without coupling with any pre-treatment tools.",
keywords = "Ambient ionization, Environmental analysis, Steroids hormone, Wastewater",
author = "Lei, {Y. T.} and Y. Lu and Zhang, {Tian C} and Y. Qi and Yongfeng Lu",
year = "2016",
month = "6",
day = "1",
doi = "10.1007/s12665-016-5830-z",
language = "English (US)",
volume = "75",
journal = "Environmental Earth Sciences",
issn = "1866-6280",
publisher = "Springer Verlag",
number = "12",

}

TY - JOUR

T1 - Rapid screening of testosterone in the aquatic environment using direct analysis in real-time (DART) mass spectrometry

AU - Lei, Y. T.

AU - Lu, Y.

AU - Zhang, Tian C

AU - Qi, Y.

AU - Lu, Yongfeng

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Testosterone (TES) in the aquatic environment was analyzed for the first time using direct analysis in real-time mass spectrometry (DART MS) without any sample pre-treatment. TES with the addition of 2H and its dehydration products was observed. The instrument parameters were studied systematically and optimized concerning the specific analyte. Limits of detection for the pure compound were 2.5 ng for TES spiked in raw wastewater samples and 500 pg in TES standards. The whole analysis time was within 1 min. A simple derivatization of TES by hydroxylamine and introducing ammonia as dopant gas for the sensitivity and intensity enhancement were also investigated. Both methods improved the intensity of the TES signal by about twofold. The results of this study illustrate that DART MS is capable to detect TES in complicated matrixes and could realize the rapid and direct screening of TES in the aquatic environment at a low nanogram range for pure compound without coupling with any pre-treatment tools.

AB - Testosterone (TES) in the aquatic environment was analyzed for the first time using direct analysis in real-time mass spectrometry (DART MS) without any sample pre-treatment. TES with the addition of 2H and its dehydration products was observed. The instrument parameters were studied systematically and optimized concerning the specific analyte. Limits of detection for the pure compound were 2.5 ng for TES spiked in raw wastewater samples and 500 pg in TES standards. The whole analysis time was within 1 min. A simple derivatization of TES by hydroxylamine and introducing ammonia as dopant gas for the sensitivity and intensity enhancement were also investigated. Both methods improved the intensity of the TES signal by about twofold. The results of this study illustrate that DART MS is capable to detect TES in complicated matrixes and could realize the rapid and direct screening of TES in the aquatic environment at a low nanogram range for pure compound without coupling with any pre-treatment tools.

KW - Ambient ionization

KW - Environmental analysis

KW - Steroids hormone

KW - Wastewater

UR - http://www.scopus.com/inward/record.url?scp=84975882069&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84975882069&partnerID=8YFLogxK

U2 - 10.1007/s12665-016-5830-z

DO - 10.1007/s12665-016-5830-z

M3 - Article

VL - 75

JO - Environmental Earth Sciences

JF - Environmental Earth Sciences

SN - 1866-6280

IS - 12

M1 - 1005

ER -