Rapid-scan EPR of immobilized nitroxides

Zhelin Yu, Richard W. Quine, George A. Rinard, Mark Tseitlin, Hanan Elajaili, Velavan Kathirvelu, Laura J. Clouston, Przemysław J. Boratyński, Andrzej Rajca, Richard Stein, Hassane McHaourab, Sandra S. Eaton, Gareth R. Eaton

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

X-band electron paramagnetic resonance spectra of immobilized nitroxides were obtained by rapid scan at 293 K. Scan widths were 155 G with 13.4 kHz scan frequency for 14N-perdeuterated tempone and for T4 lysozyme doubly spin labeled with an iodoacetamide spirocyclohexyl nitroxide and 100 G with 20.9 kHz scan frequency for 15N-perdeuterated tempone. These wide scans were made possible by modifications to our rapid-scan driver, scan coils made of Litz wire, and the placement of highly conducting aluminum plates on the poles of a Bruker 10″ magnet to reduce resistive losses in the magnet pole faces. For the same data acquisition time, the signal-to-noise for the rapid-scan absorption spectra was about an order of magnitude higher than for continuous wave first-derivative spectra recorded with modulation amplitudes that do not broaden the lineshapes.

Original languageEnglish (US)
Pages (from-to)67-71
Number of pages5
JournalJournal of Magnetic Resonance
Volume247
DOIs
StatePublished - Oct 2014

Fingerprint

Triacetoneamine-N-Oxyl
Magnets
Paramagnetic resonance
Poles
poles
magnets
Iodoacetamide
Amplitude modulation
lysozyme
Electron Spin Resonance Spectroscopy
Muramidase
superhigh frequencies
Aluminum
data acquisition
continuous radiation
Noise
Absorption spectra
Data acquisition
electron paramagnetic resonance
coils

Keywords

  • EPR Sucrose octaacetate glass
  • Immobilized nitroxide
  • Litz wire coils
  • Rapid scan
  • Trehalose glass

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

Cite this

Yu, Z., Quine, R. W., Rinard, G. A., Tseitlin, M., Elajaili, H., Kathirvelu, V., ... Eaton, G. R. (2014). Rapid-scan EPR of immobilized nitroxides. Journal of Magnetic Resonance, 247, 67-71. https://doi.org/10.1016/j.jmr.2014.08.008

Rapid-scan EPR of immobilized nitroxides. / Yu, Zhelin; Quine, Richard W.; Rinard, George A.; Tseitlin, Mark; Elajaili, Hanan; Kathirvelu, Velavan; Clouston, Laura J.; Boratyński, Przemysław J.; Rajca, Andrzej; Stein, Richard; McHaourab, Hassane; Eaton, Sandra S.; Eaton, Gareth R.

In: Journal of Magnetic Resonance, Vol. 247, 10.2014, p. 67-71.

Research output: Contribution to journalArticle

Yu, Z, Quine, RW, Rinard, GA, Tseitlin, M, Elajaili, H, Kathirvelu, V, Clouston, LJ, Boratyński, PJ, Rajca, A, Stein, R, McHaourab, H, Eaton, SS & Eaton, GR 2014, 'Rapid-scan EPR of immobilized nitroxides', Journal of Magnetic Resonance, vol. 247, pp. 67-71. https://doi.org/10.1016/j.jmr.2014.08.008
Yu Z, Quine RW, Rinard GA, Tseitlin M, Elajaili H, Kathirvelu V et al. Rapid-scan EPR of immobilized nitroxides. Journal of Magnetic Resonance. 2014 Oct;247:67-71. https://doi.org/10.1016/j.jmr.2014.08.008
Yu, Zhelin ; Quine, Richard W. ; Rinard, George A. ; Tseitlin, Mark ; Elajaili, Hanan ; Kathirvelu, Velavan ; Clouston, Laura J. ; Boratyński, Przemysław J. ; Rajca, Andrzej ; Stein, Richard ; McHaourab, Hassane ; Eaton, Sandra S. ; Eaton, Gareth R. / Rapid-scan EPR of immobilized nitroxides. In: Journal of Magnetic Resonance. 2014 ; Vol. 247. pp. 67-71.
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abstract = "X-band electron paramagnetic resonance spectra of immobilized nitroxides were obtained by rapid scan at 293 K. Scan widths were 155 G with 13.4 kHz scan frequency for 14N-perdeuterated tempone and for T4 lysozyme doubly spin labeled with an iodoacetamide spirocyclohexyl nitroxide and 100 G with 20.9 kHz scan frequency for 15N-perdeuterated tempone. These wide scans were made possible by modifications to our rapid-scan driver, scan coils made of Litz wire, and the placement of highly conducting aluminum plates on the poles of a Bruker 10″ magnet to reduce resistive losses in the magnet pole faces. For the same data acquisition time, the signal-to-noise for the rapid-scan absorption spectra was about an order of magnitude higher than for continuous wave first-derivative spectra recorded with modulation amplitudes that do not broaden the lineshapes.",
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