Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets

Robert V. Farese, Paul E. Dimarco, Demeteria E. Barnes, Mohammad A. Sabir, Ronald E. Larson, John S Davis, Anthony D. Morrison

Research output: Contribution to journalArticle

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Abstract

Glucose effects on islet phospholipids were examined during direct incubation or after 3 days of 32P prelabeling in primary culture. In both cases, glucose increased the 32P content of phosphatidic acid (PA), phosphatidylinositol (PI), and polyphosphoinositides (PPI). Glucose-induced increases in PA, PI, and PPI in the culture-prelabeling experiments were evident within 1 min, dose related, and reflective of increases in phospholipid mass, which was confirmed in direct incubations by measurement of PI phosphorus. Thus, in addition to increasing PI-PPI hydrolysis, glucose increases de novo phospholipid synthesis in pancreatic islets. The latter may result from enhanced glycolysis and substrate availability for PA-PI-PPI synthesis, since glyceraldehyde and pyruvic acid also increased PI levels. Our findings raise the possibility that increases in PA, PI, and PPI synthesis could serve as a mechanism to enhance the generation of intracellular mediators, which are purported to regulate insulin secretion.

Original languageEnglish (US)
Pages (from-to)1498-1503
Number of pages6
JournalEndocrinology
Volume118
Issue number4
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

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Phosphatidic Acids
Phosphatidylinositols
Islets of Langerhans
Phosphatidylinositol Phosphates
Glucose
Phospholipids
Glyceraldehyde
Glycolysis
Pyruvic Acid
Phosphorus
Hydrolysis
Insulin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Farese, R. V., Dimarco, P. E., Barnes, D. E., Sabir, M. A., Larson, R. E., Davis, J. S., & Morrison, A. D. (1986). Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets. Endocrinology, 118(4), 1498-1503. https://doi.org/10.1210/endo-118-4-1498

Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets. / Farese, Robert V.; Dimarco, Paul E.; Barnes, Demeteria E.; Sabir, Mohammad A.; Larson, Ronald E.; Davis, John S; Morrison, Anthony D.

In: Endocrinology, Vol. 118, No. 4, 01.01.1986, p. 1498-1503.

Research output: Contribution to journalArticle

Farese, RV, Dimarco, PE, Barnes, DE, Sabir, MA, Larson, RE, Davis, JS & Morrison, AD 1986, 'Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets', Endocrinology, vol. 118, no. 4, pp. 1498-1503. https://doi.org/10.1210/endo-118-4-1498
Farese, Robert V. ; Dimarco, Paul E. ; Barnes, Demeteria E. ; Sabir, Mohammad A. ; Larson, Ronald E. ; Davis, John S ; Morrison, Anthony D. / Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets. In: Endocrinology. 1986 ; Vol. 118, No. 4. pp. 1498-1503.
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