Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets

Robert V. Farese, Paul E. Dimarco, Demeteria E. Barnes, Mohammad A. Sabir, Ronald E. Larson, John S. Davis, Anthony D. Morrison

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Abstract

Glucose effects on islet phospholipids were examined during direct incubation or after 3 days of 32P prelabeling in primary culture. In both cases, glucose increased the 32P content of phosphatidic acid (PA), phosphatidylinositol (PI), and polyphosphoinositides (PPI). Glucose-induced increases in PA, PI, and PPI in the culture-prelabeling experiments were evident within 1 min, dose related, and reflective of increases in phospholipid mass, which was confirmed in direct incubations by measurement of PI phosphorus. Thus, in addition to increasing PI-PPI hydrolysis, glucose increases de novo phospholipid synthesis in pancreatic islets. The latter may result from enhanced glycolysis and substrate availability for PA-PI-PPI synthesis, since glyceraldehyde and pyruvic acid also increased PI levels. Our findings raise the possibility that increases in PA, PI, and PPI synthesis could serve as a mechanism to enhance the generation of intracellular mediators, which are purported to regulate insulin secretion.

Original languageEnglish (US)
Pages (from-to)1498-1503
Number of pages6
JournalEndocrinology
Volume118
Issue number4
DOIs
StatePublished - Jan 1 1986

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Phosphatidic Acids
Phosphatidylinositols
Islets of Langerhans
Phosphatidylinositol Phosphates
Glucose
Phospholipids
Glyceraldehyde
Glycolysis
Pyruvic Acid
Phosphorus
Hydrolysis
Insulin

ASJC Scopus subject areas

  • Endocrinology

Cite this

Farese, R. V., Dimarco, P. E., Barnes, D. E., Sabir, M. A., Larson, R. E., Davis, J. S., & Morrison, A. D. (1986). Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets. Endocrinology, 118(4), 1498-1503. https://doi.org/10.1210/endo-118-4-1498

Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets. / Farese, Robert V.; Dimarco, Paul E.; Barnes, Demeteria E.; Sabir, Mohammad A.; Larson, Ronald E.; Davis, John S.; Morrison, Anthony D.

In: Endocrinology, Vol. 118, No. 4, 01.01.1986, p. 1498-1503.

Research output: Contribution to journalArticle

Farese, RV, Dimarco, PE, Barnes, DE, Sabir, MA, Larson, RE, Davis, JS & Morrison, AD 1986, 'Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets', Endocrinology, vol. 118, no. 4, pp. 1498-1503. https://doi.org/10.1210/endo-118-4-1498
Farese, Robert V. ; Dimarco, Paul E. ; Barnes, Demeteria E. ; Sabir, Mohammad A. ; Larson, Ronald E. ; Davis, John S. ; Morrison, Anthony D. / Rapid glucose-dependent increases in phosphatidic acid and phosphoinositides in rat pancreatic islets. In: Endocrinology. 1986 ; Vol. 118, No. 4. pp. 1498-1503.
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