Rapid fingerprinting of milk thermal processing history by intact protein mass spectrometry with nondenaturing chromatography

Philip E Johnson, Mark Philo, Andrew Watson, E. N.Clare Mills

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Thermal processing of foods results in proteins undergoing conformational changes, aggregation, and chemical modification notably with sugars via the Maillard reaction. This can impact their functional, nutritional, and allergenic properties. Native size-exclusion chromatography with online electrospray mass spectrometry (SEC-ESI-MS) was used to characterize processing-induced changes in milk proteins in a range of milk products. Milk products could be readily grouped into either pasteurized liquid milks, heavily processed milks, or milk powders by SEC behavior, particularly by aggregation of whey proteins by thermal processing. Maillard modification of all major milk proteins by lactose was observed by MS and was primarily present in milk powders. The method developed is a rapid tool for fingerprinting the processing history of milk and has potential as a quality control method for food ingredient manufacture. The method described here can profile milk protein oligomeric state, aggregation, and Maillard modification in a single shot, rapid analysis.

Original languageEnglish (US)
Pages (from-to)12420-12427
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume59
Issue number23
DOIs
StatePublished - Dec 14 2011

Fingerprint

Chromatography
Mass spectrometry
Mass Spectrometry
chromatography
Milk
Hot Temperature
History
dried milk
milk proteins
heat treatment
mass spectrometry
milk
history
dairy products
Milk Proteins
Proteins
proteins
Maillard reaction
whey protein
Agglomeration

Keywords

  • Maillard
  • Milk proteins
  • casein
  • electrospray
  • lactose
  • processing
  • α-lactalbumin
  • β-lactoglobulin

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Rapid fingerprinting of milk thermal processing history by intact protein mass spectrometry with nondenaturing chromatography. / Johnson, Philip E; Philo, Mark; Watson, Andrew; Mills, E. N.Clare.

In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 23, 14.12.2011, p. 12420-12427.

Research output: Contribution to journalArticle

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