Rapid calibration of near-infrared spectroscopic measurements of mammalian cell cultivations

Mark R. Riley, Carl D. Okeson, Brenna L. Frazier

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Near-infrared (NIR) spectroscopy is a flexible method that can be employed to noninvasively monitor the concentrations of multiple nutrients and wastes in mammalian cell bioreactors. Development of suitable calibrations can be a labor- and time-intensive process that must be repeated when process conditions are altered significantly. To address this difficulty, we have produced a new approach for generating NIR spectroscopic calibrations that requires significantly less time compared with standard calibration schemes. This method reduces development time from the present level of several weeks to several hours. A small number of experimentally collected spectra serve as inputs to a computational procedure that yields a large number of simulated spectra, each containing both analyte-specific and analyte-independent information. Such simulated spectra may be employed as a calibration set for quantifying analytes in experimentally collected spectra. Spectroscopic measurements of the concentrations of five components (ammonia, glucose, glutamate, glutamine, and lactate) can be accomplished with levels of error similar to those obtained with full experimental calibrations. A key to this process is the utilization of random numbers, which randomizes the influence of natural variations, present in each experimentally collected spectrum, on the resultant composite spectrum. This approach may increase the feasibility of employing NIR spectroscopy to monitor bioreactors and other biological processes subjected to varying operating conditions.

Original languageEnglish (US)
Pages (from-to)1133-1141
Number of pages9
JournalBiotechnology Progress
Volume15
Issue number6
DOIs
StatePublished - Nov 1 1999

Fingerprint

Calibration
calibration
Near-Infrared Spectroscopy
near-infrared spectroscopy
Bioreactors
bioreactors
cells
Biological Phenomena
monitoring
Glutamine
Ammonia
glutamine
glutamates
lactates
Glutamic Acid
Lactic Acid
labor
ammonia
Glucose
Food

ASJC Scopus subject areas

  • Biotechnology

Cite this

Rapid calibration of near-infrared spectroscopic measurements of mammalian cell cultivations. / Riley, Mark R.; Okeson, Carl D.; Frazier, Brenna L.

In: Biotechnology Progress, Vol. 15, No. 6, 01.11.1999, p. 1133-1141.

Research output: Contribution to journalArticle

Riley, Mark R. ; Okeson, Carl D. ; Frazier, Brenna L. / Rapid calibration of near-infrared spectroscopic measurements of mammalian cell cultivations. In: Biotechnology Progress. 1999 ; Vol. 15, No. 6. pp. 1133-1141.
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