Rapid and reliable quantitation of amino acids and myo-inositol in mouse brain by high performance liquid chromatography and tandem mass spectrometry

Sai P. Bathena, Jiangeng Huang, Adrian A. Epstein, Howard Eliot Gendelman, Michael D. Boska, Yazen Alnouti

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15. min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20. ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000. ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume893-894
DOIs
StatePublished - Apr 15 2012

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High performance liquid chromatography
Biomarkers
Inositol
Tandem Mass Spectrometry
Chromatography
Aspartic Acid
Mass spectrometry
Brain
High Pressure Liquid Chromatography
Neurodegenerative diseases
Aminobutyrates
Amino Acids
Phosphorylcholine
Creatine
Taurine
Liquid chromatography
Choline
Glutamine
Silicon Dioxide
Methanol

Keywords

  • Amino acids
  • HILIC
  • LC-MS/MS
  • Multiple reaction monitoring (MRM)
  • Myo-inositol

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

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abstract = "Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15. min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20. ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000. ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.",
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AU - Bathena, Sai P.

AU - Huang, Jiangeng

AU - Epstein, Adrian A.

AU - Gendelman, Howard Eliot

AU - Boska, Michael D.

AU - Alnouti, Yazen

PY - 2012/4/15

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N2 - Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15. min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20. ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000. ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.

AB - Amino acids and myo-inositol have long been proposed as putative biomarkers for neurodegenerative diseases. Accurate measures and stability have precluded their selective use. To this end, a sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method based on multiple reaction monitoring was developed to simultaneously quantify glutamine, glutamate, γ-aminobutyric acid (GABA), aspartic acid, N-acetyl aspartic acid, taurine, choline, creatine, phosphocholine and myo-inositol in mouse brain by methanol extractions. Chromatography was performed using a hydrophilic interaction chromatography silica column within in a total run time of 15. min. The validated method is selective, sensitive, accurate, and precise. The method has a limit of quantification ranging from 2.5 to 20. ng/ml for a range of analytes and a dynamic range from 2.5-20 to 500-4000. ng/ml. This LC-MS/MS method was validated for biomarker discovery in models of human neurological disorders.

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