Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection

Ryan J. Ebmeier, George Gogos, Scott E. Whitney, Amitabha Sarkar, Hendrik J Viljoen, Michael Nelson, Nisha V. Padhye

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The use of Ranque-Hilsch Vortex Tube Thermocycler for 96 bp λ-DNA amplicon and real-time optical detection was discussed. The speed of the Ranque-Hilsch Vortex Tube Thermocycler was best utilized in combination with a fast enzyme such as KOD Hot Start Polymerase. It was found that the total reaction time which includes the 30 second hot start and subsequent 45 cycles between the denaturation, annealing and elongation temperatures, was less than 12 minutes. The results show that the 0.25 fg DNA sample surpassed the threshold value 8 minutes into the reaction at cycle number 34.

Original languageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages10479-10485
Number of pages7
StatePublished - 2004
Event2004 AIChE Annual Meeting - Austin, TX
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CityAustin, TX
Period11/7/0411/12/04

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Amplification
Vortex flow
DNA
Denaturation
Elongation
Enzymes
Annealing
Temperature

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Ebmeier, R. J., Gogos, G., Whitney, S. E., Sarkar, A., Viljoen, H. J., Nelson, M., & Padhye, N. V. (2004). Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection. In AIChE Annual Meeting, Conference Proceedings (pp. 10479-10485). [554e]

Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection. / Ebmeier, Ryan J.; Gogos, George; Whitney, Scott E.; Sarkar, Amitabha; Viljoen, Hendrik J; Nelson, Michael; Padhye, Nisha V.

AIChE Annual Meeting, Conference Proceedings. 2004. p. 10479-10485 554e.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ebmeier, RJ, Gogos, G, Whitney, SE, Sarkar, A, Viljoen, HJ, Nelson, M & Padhye, NV 2004, Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection. in AIChE Annual Meeting, Conference Proceedings., 554e, pp. 10479-10485, 2004 AIChE Annual Meeting, Austin, TX, 11/7/04.
Ebmeier RJ, Gogos G, Whitney SE, Sarkar A, Viljoen HJ, Nelson M et al. Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection. In AIChE Annual Meeting, Conference Proceedings. 2004. p. 10479-10485. 554e
Ebmeier, Ryan J. ; Gogos, George ; Whitney, Scott E. ; Sarkar, Amitabha ; Viljoen, Hendrik J ; Nelson, Michael ; Padhye, Nisha V. / Ranque-Hilsch vortex tube thermocycler for fast DNA amplification and real-time optical detection. AIChE Annual Meeting, Conference Proceedings. 2004. pp. 10479-10485
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