Ranque-hilsch vortex tube thermocycler for DNA amplification

R. Ebmeier, S. Whitney, S. Alugupally, M. Nelson, N. Padhye, George Gogos, Hendrik J Viljoen

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An innovative polymerase chain reaction (PCR) thermocycler using pressurized gas through a Ranque-Hilsch vortex tube is described below. This device can amplify 10pg of 186bp Escherichia coli uidA amplicon in a 20 μL sample 3.3 × 108-fold, by performing 35 cycles in less than 8 min. This PCR amplification corresponds to an overall efficiency of 75%.

Original languageEnglish (US)
Pages (from-to)567-570
Number of pages4
JournalInstrumentation Science and Technology
Volume32
Issue number5
DOIs
StatePublished - Sep 1 2004

Fingerprint

vortex tubes
polymerase chain reaction
Polymerase chain reaction
Amplification
vortex
amplification
Vortex flow
DNA
deoxyribonucleic acid
Escherichia
Escherichia coli
Gases
fold
cycles
gases
gas

Keywords

  • DNA amplification
  • PCR method
  • Reaction time
  • Thermocycles

ASJC Scopus subject areas

  • Analytical Chemistry
  • Instrumentation

Cite this

Ranque-hilsch vortex tube thermocycler for DNA amplification. / Ebmeier, R.; Whitney, S.; Alugupally, S.; Nelson, M.; Padhye, N.; Gogos, George; Viljoen, Hendrik J.

In: Instrumentation Science and Technology, Vol. 32, No. 5, 01.09.2004, p. 567-570.

Research output: Contribution to journalArticle

Ebmeier, R. ; Whitney, S. ; Alugupally, S. ; Nelson, M. ; Padhye, N. ; Gogos, George ; Viljoen, Hendrik J. / Ranque-hilsch vortex tube thermocycler for DNA amplification. In: Instrumentation Science and Technology. 2004 ; Vol. 32, No. 5. pp. 567-570.
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