Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells: A comparison with 5-[125I]iodo-2'-deoxyuridine

G. M. Makrigiorgos, A. I. Kassis, J. Baranowska-Kortylewicz, K. D. McElvany, M. J. Welch, K. S.R. Sastry, S. J. Adelstein

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The toxic effects of the short-lived (T( 1/2 ) = 13.2 h) Auger-electron-emitting isotope 123I, incorporated in the form of 123IUdR into the DNA of V79 cells in vitro, have been investigated and compared to those of 125IUdR. For the concentrations tested, the rate of incorporation of 123IUdR at any time is proportional to the concentration of extracellular radioactivity. The curve for survival of clonogenic cells decreases exponentially and exhibits no shoulder at low doses. The mean lethal dose (D37) to the nucleus is 79 ± 9 cGy and is about the same as that obtained previously with 125IUdR. However, the total number of decays needed to produce this D37 with 123IUdR is about twice that required with 125IUdR, approximately equal to the ratio of the energy deposited in microscopic volumes by 125I and 123I, respectively. This correlation suggests that nuclear recoil, electronic excitation, and chemical transmutation are probably of minor importance to the observed biological toxicity with either isotope. The results also indicate that there are no saturation effects in the decay of 125IUdR in the DNA of V79 cells (i.e., all of the emitted energy is biologically effective) and that each of the two steps involved in the 125I decay is equally effective in causing biological damage.

Original languageEnglish (US)
Pages (from-to)532-544
Number of pages13
JournalRadiation Research
Volume118
Issue number3
DOIs
StatePublished - Jan 1 1989

Fingerprint

Idoxuridine
Isotopes
deterioration
Poisons
DNA
isotopes
decay
deoxyribonucleic acid
cells
Radioactivity
dosage
Cell Survival
transmutation
lethal dose
energy
Electrons
shoulders
radioactivity
toxicity
electronics

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Makrigiorgos, G. M., Kassis, A. I., Baranowska-Kortylewicz, J., McElvany, K. D., Welch, M. J., Sastry, K. S. R., & Adelstein, S. J. (1989). Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells: A comparison with 5-[125I]iodo-2'-deoxyuridine. Radiation Research, 118(3), 532-544. https://doi.org/10.2307/3577411

Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells : A comparison with 5-[125I]iodo-2'-deoxyuridine. / Makrigiorgos, G. M.; Kassis, A. I.; Baranowska-Kortylewicz, J.; McElvany, K. D.; Welch, M. J.; Sastry, K. S.R.; Adelstein, S. J.

In: Radiation Research, Vol. 118, No. 3, 01.01.1989, p. 532-544.

Research output: Contribution to journalArticle

Makrigiorgos, GM, Kassis, AI, Baranowska-Kortylewicz, J, McElvany, KD, Welch, MJ, Sastry, KSR & Adelstein, SJ 1989, 'Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells: A comparison with 5-[125I]iodo-2'-deoxyuridine', Radiation Research, vol. 118, no. 3, pp. 532-544. https://doi.org/10.2307/3577411
Makrigiorgos, G. M. ; Kassis, A. I. ; Baranowska-Kortylewicz, J. ; McElvany, K. D. ; Welch, M. J. ; Sastry, K. S.R. ; Adelstein, S. J. / Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells : A comparison with 5-[125I]iodo-2'-deoxyuridine. In: Radiation Research. 1989 ; Vol. 118, No. 3. pp. 532-544.
@article{b96c2a65d626466aaf6614ff234085a1,
title = "Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells: A comparison with 5-[125I]iodo-2'-deoxyuridine",
abstract = "The toxic effects of the short-lived (T( 1/2 ) = 13.2 h) Auger-electron-emitting isotope 123I, incorporated in the form of 123IUdR into the DNA of V79 cells in vitro, have been investigated and compared to those of 125IUdR. For the concentrations tested, the rate of incorporation of 123IUdR at any time is proportional to the concentration of extracellular radioactivity. The curve for survival of clonogenic cells decreases exponentially and exhibits no shoulder at low doses. The mean lethal dose (D37) to the nucleus is 79 ± 9 cGy and is about the same as that obtained previously with 125IUdR. However, the total number of decays needed to produce this D37 with 123IUdR is about twice that required with 125IUdR, approximately equal to the ratio of the energy deposited in microscopic volumes by 125I and 123I, respectively. This correlation suggests that nuclear recoil, electronic excitation, and chemical transmutation are probably of minor importance to the observed biological toxicity with either isotope. The results also indicate that there are no saturation effects in the decay of 125IUdR in the DNA of V79 cells (i.e., all of the emitted energy is biologically effective) and that each of the two steps involved in the 125I decay is equally effective in causing biological damage.",
author = "Makrigiorgos, {G. M.} and Kassis, {A. I.} and J. Baranowska-Kortylewicz and McElvany, {K. D.} and Welch, {M. J.} and Sastry, {K. S.R.} and Adelstein, {S. J.}",
year = "1989",
month = "1",
day = "1",
doi = "10.2307/3577411",
language = "English (US)",
volume = "118",
pages = "532--544",
journal = "Radiation Research",
issn = "0033-7587",
publisher = "Radiation Research Society",
number = "3",

}

TY - JOUR

T1 - Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells

T2 - A comparison with 5-[125I]iodo-2'-deoxyuridine

AU - Makrigiorgos, G. M.

AU - Kassis, A. I.

AU - Baranowska-Kortylewicz, J.

AU - McElvany, K. D.

AU - Welch, M. J.

AU - Sastry, K. S.R.

AU - Adelstein, S. J.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The toxic effects of the short-lived (T( 1/2 ) = 13.2 h) Auger-electron-emitting isotope 123I, incorporated in the form of 123IUdR into the DNA of V79 cells in vitro, have been investigated and compared to those of 125IUdR. For the concentrations tested, the rate of incorporation of 123IUdR at any time is proportional to the concentration of extracellular radioactivity. The curve for survival of clonogenic cells decreases exponentially and exhibits no shoulder at low doses. The mean lethal dose (D37) to the nucleus is 79 ± 9 cGy and is about the same as that obtained previously with 125IUdR. However, the total number of decays needed to produce this D37 with 123IUdR is about twice that required with 125IUdR, approximately equal to the ratio of the energy deposited in microscopic volumes by 125I and 123I, respectively. This correlation suggests that nuclear recoil, electronic excitation, and chemical transmutation are probably of minor importance to the observed biological toxicity with either isotope. The results also indicate that there are no saturation effects in the decay of 125IUdR in the DNA of V79 cells (i.e., all of the emitted energy is biologically effective) and that each of the two steps involved in the 125I decay is equally effective in causing biological damage.

AB - The toxic effects of the short-lived (T( 1/2 ) = 13.2 h) Auger-electron-emitting isotope 123I, incorporated in the form of 123IUdR into the DNA of V79 cells in vitro, have been investigated and compared to those of 125IUdR. For the concentrations tested, the rate of incorporation of 123IUdR at any time is proportional to the concentration of extracellular radioactivity. The curve for survival of clonogenic cells decreases exponentially and exhibits no shoulder at low doses. The mean lethal dose (D37) to the nucleus is 79 ± 9 cGy and is about the same as that obtained previously with 125IUdR. However, the total number of decays needed to produce this D37 with 123IUdR is about twice that required with 125IUdR, approximately equal to the ratio of the energy deposited in microscopic volumes by 125I and 123I, respectively. This correlation suggests that nuclear recoil, electronic excitation, and chemical transmutation are probably of minor importance to the observed biological toxicity with either isotope. The results also indicate that there are no saturation effects in the decay of 125IUdR in the DNA of V79 cells (i.e., all of the emitted energy is biologically effective) and that each of the two steps involved in the 125I decay is equally effective in causing biological damage.

UR - http://www.scopus.com/inward/record.url?scp=0024474962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024474962&partnerID=8YFLogxK

U2 - 10.2307/3577411

DO - 10.2307/3577411

M3 - Article

C2 - 2727274

AN - SCOPUS:0024474962

VL - 118

SP - 532

EP - 544

JO - Radiation Research

JF - Radiation Research

SN - 0033-7587

IS - 3

ER -