Radioligand binding methods for membrane preparations and intact cells

David B. Bylund, Myron Lee Toews

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The radioligand binding assay is a relatively simple but powerful tool for studying G protein-coupled receptors. There are three basic types of radioligand binding experiments: (1) saturation experiments from which the affinity of the radioligand for the receptor and the binding site density can be determined; (2) inhibition experiments from which the affinity of a competing, unlabeled compound for the receptor can be determined; and (3) kinetic experiments from which the forward and reverse rate constants for radioligand binding can be determined. Detailed methods for typical radioligand binding assays for G protein-coupled receptors in membranes and intact cells are presented for these types of experiments. Detailed procedures for analysis of the data obtained from these experiments are also given.

Original languageEnglish (US)
Pages (from-to)135-164
Number of pages30
JournalMethods in Molecular Biology
Volume746
DOIs
StatePublished - 2011

Fingerprint

Radioligand Assay
G-Protein-Coupled Receptors
Membranes
Binding Sites
Cell Membrane

Keywords

  • Affinity
  • Assay
  • Binding
  • Competition
  • G protein-coupled receptor
  • Inhibition
  • Intact cell
  • Kinetic
  • Non-specific binding
  • Radioligand
  • Radioreceptor
  • Rate constant
  • Receptor
  • Saturation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Radioligand binding methods for membrane preparations and intact cells. / Bylund, David B.; Toews, Myron Lee.

In: Methods in Molecular Biology, Vol. 746, 2011, p. 135-164.

Research output: Contribution to journalArticle

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