Quorum-sensing control of biofilm factors in Staphylococcus epidermidis

Cuong Vuong, Christiane Gerke, Greg A. Somerville, Elizabeth R. Fischer, Michael Otto

Research output: Contribution to journalArticle

228 Citations (Scopus)

Abstract

Staphylococcus epidermidis is the most frequent cause of nosocomial sepsis and catheter-related infections, in which biofilm formation is considered to be the main virulence mechanism. Quorum-sensing systems have been recognized as important regulators of virulence and biofilm formation in many bacteria. There is a single quorum-sensing system in S. epidermidis encoded by the agr operon. To investigate quorum-sensing control of biofilm formation, we constructed an agr deletion mutant, assayed for the different stages of biofilm formation, and determined agr-dependent regulation of biofilm factors. The agr mutant showed increased biofilm formation, primary attachment, and expression of the autolysin AtlE, but lacked δ-toxin production. However, the level of polysaccharide intercellular adhesin expression was equivalent to the isogenic wild-type strain. In contrast to AtlE, which is known to influence primary attachment, δ-toxin appeared to exert its effect on attachment to polystyrene during later stages of biofilm formation. Importantly, addition of cross-inhibiting pheromones mimicked an agr mutation and significantly enhanced biofilm formation, which suggests that care should be used when treating S. epidermidis infections with cross-inhibiting peptides. Our data demonstrate the importance of quorum sensing in the establishment of a biofilm in this critical human pathogen.

Original languageEnglish (US)
Pages (from-to)706-718
Number of pages13
JournalJournal of Infectious Diseases
Volume188
Issue number5
DOIs
StatePublished - Sep 1 2003

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Quorum Sensing
Staphylococcus epidermidis
Biofilms
Virulence
N-Acetylmuramoyl-L-alanine Amidase
Catheter-Related Infections
Pheromones
Polystyrenes
Operon
Cross Infection
Sepsis
Bacteria
Peptides
Mutation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Quorum-sensing control of biofilm factors in Staphylococcus epidermidis. / Vuong, Cuong; Gerke, Christiane; Somerville, Greg A.; Fischer, Elizabeth R.; Otto, Michael.

In: Journal of Infectious Diseases, Vol. 188, No. 5, 01.09.2003, p. 706-718.

Research output: Contribution to journalArticle

Vuong, Cuong ; Gerke, Christiane ; Somerville, Greg A. ; Fischer, Elizabeth R. ; Otto, Michael. / Quorum-sensing control of biofilm factors in Staphylococcus epidermidis. In: Journal of Infectious Diseases. 2003 ; Vol. 188, No. 5. pp. 706-718.
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