Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

B. S. Coates, A. P. Alves, H. Wang, X. Zhou, T. Nowatzki, H. Chen, M. Rangasamy, H. M. Robertson, C. W. Whitfield, K. K. Walden, S. D. Kachman, B. W. French, L. J. Meinke, D. Hawthorne, C. A. Abel, T. W. Sappington, B. D. Siegfried, N. J. Miller

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases ≥ 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methyl-parathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyxesterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established.

Original languageEnglish (US)
Pages (from-to)1-15
Number of pages15
JournalInsect Molecular Biology
Volume25
Issue number1
DOIs
StatePublished - Feb 1 2016

Fingerprint

Methyl Parathion
parathion-methyl
Diabrotica virgifera virgifera
Organophosphates
Quantitative Trait Loci
organophosphorus compounds
Genomics
Zea mays
quantitative trait loci
genomics
Esterases
esterases
corn
Insecticide Resistance
rootworms
organophosphorus insecticides
insecticide resistance
Insecticides
insect pests
probes (equipment)

Keywords

  • functional genomics
  • genome mapping
  • methyl-parathion resistance
  • pesticide resistance

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera. / Coates, B. S.; Alves, A. P.; Wang, H.; Zhou, X.; Nowatzki, T.; Chen, H.; Rangasamy, M.; Robertson, H. M.; Whitfield, C. W.; Walden, K. K.; Kachman, S. D.; French, B. W.; Meinke, L. J.; Hawthorne, D.; Abel, C. A.; Sappington, T. W.; Siegfried, B. D.; Miller, N. J.

In: Insect Molecular Biology, Vol. 25, No. 1, 01.02.2016, p. 1-15.

Research output: Contribution to journalArticle

Coates, BS, Alves, AP, Wang, H, Zhou, X, Nowatzki, T, Chen, H, Rangasamy, M, Robertson, HM, Whitfield, CW, Walden, KK, Kachman, SD, French, BW, Meinke, LJ, Hawthorne, D, Abel, CA, Sappington, TW, Siegfried, BD & Miller, NJ 2016, 'Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera', Insect Molecular Biology, vol. 25, no. 1, pp. 1-15. https://doi.org/10.1111/imb.12194
Coates, B. S. ; Alves, A. P. ; Wang, H. ; Zhou, X. ; Nowatzki, T. ; Chen, H. ; Rangasamy, M. ; Robertson, H. M. ; Whitfield, C. W. ; Walden, K. K. ; Kachman, S. D. ; French, B. W. ; Meinke, L. J. ; Hawthorne, D. ; Abel, C. A. ; Sappington, T. W. ; Siegfried, B. D. ; Miller, N. J. / Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera. In: Insect Molecular Biology. 2016 ; Vol. 25, No. 1. pp. 1-15.
@article{b23bbd81e95f4e408709a86993a443b2,
title = "Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera",
abstract = "The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases ≥ 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methyl-parathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyxesterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established.",
keywords = "functional genomics, genome mapping, methyl-parathion resistance, pesticide resistance",
author = "Coates, {B. S.} and Alves, {A. P.} and H. Wang and X. Zhou and T. Nowatzki and H. Chen and M. Rangasamy and Robertson, {H. M.} and Whitfield, {C. W.} and Walden, {K. K.} and Kachman, {S. D.} and French, {B. W.} and Meinke, {L. J.} and D. Hawthorne and Abel, {C. A.} and Sappington, {T. W.} and Siegfried, {B. D.} and Miller, {N. J.}",
year = "2016",
month = "2",
day = "1",
doi = "10.1111/imb.12194",
language = "English (US)",
volume = "25",
pages = "1--15",
journal = "Insect Molecular Biology",
issn = "0962-1075",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Quantitative trait locus mapping and functional genomics of an organophosphate resistance trait in the western corn rootworm, Diabrotica virgifera virgifera

AU - Coates, B. S.

AU - Alves, A. P.

AU - Wang, H.

AU - Zhou, X.

AU - Nowatzki, T.

AU - Chen, H.

AU - Rangasamy, M.

AU - Robertson, H. M.

AU - Whitfield, C. W.

AU - Walden, K. K.

AU - Kachman, S. D.

AU - French, B. W.

AU - Meinke, L. J.

AU - Hawthorne, D.

AU - Abel, C. A.

AU - Sappington, T. W.

AU - Siegfried, B. D.

AU - Miller, N. J.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases ≥ 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methyl-parathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyxesterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established.

AB - The western corn rootworm, Diabrotica virgifera virgifera, is an insect pest of corn and population suppression with chemical insecticides is an important management tool. Traits conferring organophosphate insecticide resistance have increased in frequency amongst D. v. virgifera populations, resulting in the reduced efficacy in many corn-growing regions of the USA. We used comparative functional genomic and quantitative trait locus (QTL) mapping approaches to investigate the genetic basis of D. v. virgifera resistance to the organophosphate methyl-parathion. RNA from adult methyl-parathion resistant and susceptible adults was hybridized to 8331 microarray probes. The results predicted that 11 transcripts were significantly up-regulated in resistant phenotypes, with the most significant (fold increases ≥ 2.43) being an α-esterase-like transcript. Differential expression was validated only for the α-esterase (ST020027A20C03), with 11- to 13-fold greater expression in methyl-parathion resistant adults (P < 0.05). Progeny with a segregating methyl-parathion resistance trait were obtained from a reciprocal backcross design. QTL analyses of high-throughput single nucleotide polymorphism genotype data predicted involvement of a single genome interval. These data suggest that a specific carboyxesterase may function in field-evolved corn rootworm resistance to organophosphates, even though direct linkage between the QTL and this locus could not be established.

KW - functional genomics

KW - genome mapping

KW - methyl-parathion resistance

KW - pesticide resistance

UR - http://www.scopus.com/inward/record.url?scp=84957107735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957107735&partnerID=8YFLogxK

U2 - 10.1111/imb.12194

DO - 10.1111/imb.12194

M3 - Article

C2 - 26566705

AN - SCOPUS:84957107735

VL - 25

SP - 1

EP - 15

JO - Insect Molecular Biology

JF - Insect Molecular Biology

SN - 0962-1075

IS - 1

ER -