Quantitative tissue spectroscopy of near infrared fluorescent nanosensor implants

Nicole M Iverson, Gili Bisker, Edgardo Farias, Vsevolod Ivanov, Jiyoung Ahn, Gerald N. Wogan, Michael S. Strano

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Implantable, near infrared (nIR) fluorescent nanosensors are advantageous for in vivo monitoring of biological analytes since they can be rendered selective for a particular target molecule while utilizing their unique optical properties and the nIR tissue transparency window for information transfer without an internal power source or telemetry. However, basic questions remain regarDing the optimal encapsulation platform, geometrical properties, and concentration ranges required for high signal to noise ratio and effective detection through biological tissue. In this work, we systematically explore these variables quantitatively to optimize the performance of such optical nanosensors for biomedical applications.We investigate both alginate and polyethylene glycol (PEG) as model hydrogel systems, encapsulating d(GT)15 ssDNA-wrapped singlewalled carbon nanotubes (SWNT) as model fluorescent nanoparticle sensors, responsive to riboflavin. Hydrogel sensors implanted 0.5 mm into thick tissue samples exhibit 50% reduction of initial fluorescence intensity, allowing an optical detection limit of 5.4 mm and 5.1 mm depth in tissue for alginate and PEG gels, respectively, at a SWNT concentration of 10 mg L?1, and 785 nm laser excitation of 80 mW and 30 s exposure. These findings are supported with in vivo nIR fluorescent imaging of SWNT hydrogels implanted subcutaneously in mice. For the case of SWNT, we find that the alginate system is preferable in terms of emission intensity, sensor response, rheological properties, and shelf life.

Original languageEnglish (US)
Pages (from-to)1035-1047
Number of pages13
JournalJournal of Biomedical Nanotechnology
Volume12
Issue number5
DOIs
StatePublished - May 2016

Fingerprint

Nanosensors
Carbon Nanotubes
Near-Infrared Spectroscopy
Carbon nanotubes
Alginate
Hydrogels
Spectroscopy
Tissue
Infrared radiation
Hydrogel
Polyethylene glycols
Sensors
Electric Power Supplies
Telemetry
Laser excitation
Riboflavin
Environmental Monitoring
Signal-To-Noise Ratio
Telemetering
Encapsulation

Keywords

  • Fluorescence
  • Hydrogel
  • In Vivo Biosensor
  • Nanoparticles
  • Single-Walled Carbon Nanotube

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Quantitative tissue spectroscopy of near infrared fluorescent nanosensor implants. / Iverson, Nicole M; Bisker, Gili; Farias, Edgardo; Ivanov, Vsevolod; Ahn, Jiyoung; Wogan, Gerald N.; Strano, Michael S.

In: Journal of Biomedical Nanotechnology, Vol. 12, No. 5, 05.2016, p. 1035-1047.

Research output: Contribution to journalArticle

Iverson, Nicole M ; Bisker, Gili ; Farias, Edgardo ; Ivanov, Vsevolod ; Ahn, Jiyoung ; Wogan, Gerald N. ; Strano, Michael S. / Quantitative tissue spectroscopy of near infrared fluorescent nanosensor implants. In: Journal of Biomedical Nanotechnology. 2016 ; Vol. 12, No. 5. pp. 1035-1047.
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