Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients

Michael J. Baine, Kavita Mallya, Surinder K. Batra

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

The ability of peripheral blood mononuclear cells (PBMCs) to act as a surrogate window into the presence and physiologic effects of pancreatic cancer is becoming increasingly apparent. In this chapter, we describe the techniques for isolation, lysis, RNA extraction, cDNA synthesis, and Q-RT PCR analysis of PBMCs as well as reasonable alternatives and the advantages and disadvantages of each. We further discuss the noteworthy considerations necessary for successful isolation and conversion of the high-quality PBMC RNA required to acquire interpretable and reproducible results for PBMC genetic expression analysis.

Original languageEnglish (US)
Title of host publicationPancreatic Cancer
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages157-173
Number of pages17
ISBN (Print)9781627032865
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume980
ISSN (Print)1064-3745

Fingerprint

Pancreatic Neoplasms
Real-Time Polymerase Chain Reaction
Blood Cells
RNA
Complementary DNA
Polymerase Chain Reaction

Keywords

  • Blood
  • Cancer immunity
  • Genetic expression
  • Pancreatic cancer
  • Peripheral blood mononuclear cells (PBMCs)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Baine, M. J., Mallya, K., & Batra, S. K. (2013). Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients. In Pancreatic Cancer: Methods and Protocols (pp. 157-173). (Methods in Molecular Biology; Vol. 980). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-287-2_8

Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients. / Baine, Michael J.; Mallya, Kavita; Batra, Surinder K.

Pancreatic Cancer: Methods and Protocols. Humana Press Inc., 2013. p. 157-173 (Methods in Molecular Biology; Vol. 980).

Research output: Chapter in Book/Report/Conference proceedingChapter

Baine, MJ, Mallya, K & Batra, SK 2013, Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients. in Pancreatic Cancer: Methods and Protocols. Methods in Molecular Biology, vol. 980, Humana Press Inc., pp. 157-173. https://doi.org/10.1007/978-1-62703-287-2_8
Baine MJ, Mallya K, Batra SK. Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients. In Pancreatic Cancer: Methods and Protocols. Humana Press Inc. 2013. p. 157-173. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-287-2_8
Baine, Michael J. ; Mallya, Kavita ; Batra, Surinder K. / Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients. Pancreatic Cancer: Methods and Protocols. Humana Press Inc., 2013. pp. 157-173 (Methods in Molecular Biology).
@inbook{3d66b19ebfd44c2f856ce48d847e69f1,
title = "Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients",
abstract = "The ability of peripheral blood mononuclear cells (PBMCs) to act as a surrogate window into the presence and physiologic effects of pancreatic cancer is becoming increasingly apparent. In this chapter, we describe the techniques for isolation, lysis, RNA extraction, cDNA synthesis, and Q-RT PCR analysis of PBMCs as well as reasonable alternatives and the advantages and disadvantages of each. We further discuss the noteworthy considerations necessary for successful isolation and conversion of the high-quality PBMC RNA required to acquire interpretable and reproducible results for PBMC genetic expression analysis.",
keywords = "Blood, Cancer immunity, Genetic expression, Pancreatic cancer, Peripheral blood mononuclear cells (PBMCs)",
author = "Baine, {Michael J.} and Kavita Mallya and Batra, {Surinder K.}",
year = "2013",
doi = "10.1007/978-1-62703-287-2_8",
language = "English (US)",
isbn = "9781627032865",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "157--173",
booktitle = "Pancreatic Cancer",

}

TY - CHAP

T1 - Quantitative real-time PCR expression analysis of peripheral blood mononuclear cells in pancreatic cancer patients

AU - Baine, Michael J.

AU - Mallya, Kavita

AU - Batra, Surinder K.

PY - 2013

Y1 - 2013

N2 - The ability of peripheral blood mononuclear cells (PBMCs) to act as a surrogate window into the presence and physiologic effects of pancreatic cancer is becoming increasingly apparent. In this chapter, we describe the techniques for isolation, lysis, RNA extraction, cDNA synthesis, and Q-RT PCR analysis of PBMCs as well as reasonable alternatives and the advantages and disadvantages of each. We further discuss the noteworthy considerations necessary for successful isolation and conversion of the high-quality PBMC RNA required to acquire interpretable and reproducible results for PBMC genetic expression analysis.

AB - The ability of peripheral blood mononuclear cells (PBMCs) to act as a surrogate window into the presence and physiologic effects of pancreatic cancer is becoming increasingly apparent. In this chapter, we describe the techniques for isolation, lysis, RNA extraction, cDNA synthesis, and Q-RT PCR analysis of PBMCs as well as reasonable alternatives and the advantages and disadvantages of each. We further discuss the noteworthy considerations necessary for successful isolation and conversion of the high-quality PBMC RNA required to acquire interpretable and reproducible results for PBMC genetic expression analysis.

KW - Blood

KW - Cancer immunity

KW - Genetic expression

KW - Pancreatic cancer

KW - Peripheral blood mononuclear cells (PBMCs)

UR - http://www.scopus.com/inward/record.url?scp=84879380362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879380362&partnerID=8YFLogxK

U2 - 10.1007/978-1-62703-287-2_8

DO - 10.1007/978-1-62703-287-2_8

M3 - Chapter

C2 - 23359153

AN - SCOPUS:84879380362

SN - 9781627032865

T3 - Methods in Molecular Biology

SP - 157

EP - 173

BT - Pancreatic Cancer

PB - Humana Press Inc.

ER -